Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
Keywords:
Foods genetically modified, Polymerase chain reaction, Food labelingAbstract
Detection of genetically modified organisms in the food chain is an important issue for all subjects involved in raw material control, food industry and distribution. Both labeling and traceability of genetically modified organisms are current issues that are considered for trade and regulation. Currently, labeling of genetically modified foods containing detectable transgenic material is required by the Brazilian legislation. The Brazilian government published the Decree nº 4.680 in April 2003, which requires labeling for all foods or food ingredients, with a stricter labeling threshold of 1%. Although polymerase chain reaction technology has
some limitations, the high sensitivity and specificity explain why it has been the first choice of most analytical laboratories interested in detection of genetically modified organisms and their derived products. Among the currently available methods, polymerase chain reaction-based methods are accepted, considering the sensitivity and reliability for detection of genetically modified-derived material in routine analysis. In this paper, a review of currently available polymerase chain reaction methods for screening and quantifying genetically modifiedderived ingredients is presented, discussing their applicability and limitations
References
Nodari RO, Guerra MP. Plantas transgênicas e seus produtos: impactos, riscos e segurança alimentar. Rev Nutr. 2003; 16(1):105-16.
James, C. Executive summary of global status of commercialized biotech/GM Crops: 2005. ISAAA Briefs No. 34. ISAAA [cited 2005 Mar 14]. Available from: <http://www.isaaa.org/kc/bin/briefs34/es/index.htm>.
Cavalli SB. Segurança alimentar: a abordagem dos alimentos transgênicos. Rev Nutr. 2001; 14(Supl): 41-6.
Ahmed FE. Detection of genetically modified organisms in foods. Trends in Biotechnol. 2002; 20(5):215-23.
National Research Council. Genetically modified pest-protected plants: science and regulation [cited 2002 Nov 2]. Available from: .
Schilter B, Constable A. Regulatory control of genetically modified (GM) foods: likely developments. Toxicol Letters. 2002; 127(1-3): 341-9.
Brasil. Comissão Técnica Nacional de Biossegurança. Commercial Aprovals [cited 2007 May 9]. Available from: <http://www.ctnbio.gov.br/index.php/content/view/3662.html>.
União Européia. Rastreabilidade e rotulagem dos organismos geneticamente modificados (OGM) [cited 2006 Apr 6]. Available from: <http://europa.eu.int/scadplus/leg/pt/lvb/l21170.htm>.
Van Den Eede G, Kay S, Anklam E. Analytical challenges: brindging the gap from regulation to enforcement. J AOAC Int. 2002; 85(3):757-61.
Gracia Arnaiz M. Piensando sobre el riesgo alimentario y su aceptabilidad: el caso de los alimentos transgénicos. Rev Nutr. 2004; 17(2): 125-49.
Germini A, Zanetti A, Salati C, Rossi S, Forré C, Schmid S, et al. Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J Agric Food Chem. 2004; 52(11):3275-80.
Miraglia M, Berdal KG, Brera C, Corbisier C, Holst-Jensen A, KOK A, et al. Detection and traceability of genetically modified organisms in the food production chain. Food Chem Toxicol. 2004; 42(7): 1157-80.
Tozzini AC, Martinez MC, Lucca MF, Rovere CV, Distéfano AJ, Del Vas M, et al. Semi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification. Electronic J Biotech. 2000; 3(2):1-5.
Cankar K, Ravnikar M, ZEL J, Gruden K, Toplar N. Real-time pollymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screeening assay for genetically modified organisms. J AOAC Int. 2005; 88(3):814-22.
Corbisier P, Trapmann S, Ganeberg D, Hannes L, Van Iwaarden P, Berben G, et al. Quantitative determination of roundup ready soybean (glycine max) extracted from highly processed flour. Anal Bioanal Chem. 2005; 383(2):282-90.
Feinberg M, Fernandez S, Cassard S, Charles-Delobel C. Quantification of the 35S promoter in maize DNA extracts from genetically modified organisms using real-time polymerase chain reaction: Part 2: interlaboratory study. J AOAC Int. 2005; 88(2):558-73.
Fernandez S, Charles-Delobel C, Geldreich A, Berthier G, Boyer F, Collonnier C, et al. Quantification of the 35S promoter in DNA extracts
from genetically modified organisms using realtime pollymerase chain reaction and specificity assessment on various genetically modified organisms: Part 1: Operating procedure. J AOAC Int. 2005; 88(2):547-57.
Taverniers I, Van Bockstaele E, De Loose M. Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods. Anal Bioanal Chem. 2004; 378(5):1198-207.
Weighardt F, Barbati C, Paoletti C, Querci M, Kay S, De Beuckeleer M, et al. Real-time PCR based approach for quantification of the pat gene in the T25 Zea Mays event. J AOAC Int. 2004; 87(6): 1342-55.
Greiner R, Konietzny U, Villavicencio ALCH. Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods. Food Control. 2005; 16(8):753-9.
Cardarelli P, Branquinho MR, Ferreira RTB, Cruz FP, Gemal AL. Detection of GMO in food products in Brazil: the INCQS experience. Food Control. 2005; 16(10): 859-66.
Brod FCA, Ferrari CS, Valente LL, Arisi ACM. Nested PCR detection of genetically modified soybean in soybean flour, infant formula and soymilk. LWT 2007; 40: 748-51.
Meyer R. Development and application of DNA analytical methods for the detection of GMOs in food. Food Control. 1999; 10(6):391-9.
Peano C, Samson MC, Palmieri L, Gulli M, Marmiroli N. Qualitative and quantitativeevaluation of the genomic DNA extracted from GMO and Non-GMO foodstuffs with four different extraction methods. J Agric Food Chem. 2004; 52(23):6962-8.
Terry CF, Harris N, Parkes HC. Detection of GM crops and their derivatives: critical steps in sample preparation and extraction. J AOAC Int. 2002; 85(3):768-74.
Giovannini T, Concilio L. PCR Detection of genetically modified organisms: a review. Starch. 2002; 54(8):321-7.
Holst-Jensen A, De Loose M, Van Den EEDE. Coherence between Legal requirements and approaches for detection of genetically modified organisms (GMOs) and their derived products. J Agric Food Chem. 2006; 54(8):2799-809.
Institute of Reference Materials and Measurements reference materials catalogue [cited 2006 Feb 3]. Available from: <http://www.irmm.jrc.be/>.
Chen Y, Wang Y, Ge Y, Xu B. Degradation of endogenous and exogenous genes of Roundup Ready Soybean during food processing. J Agric Food Chem. 2005; 53(26):10239-43.
Bertheau Y, Diolez A, Kobilinsky A, Magin K. Detection methods and performance criteria for genetically modified organisms. J AOAC Int. 2002; 85(3): 801-8.
Deisingh A, Badrie N. Detection approaches for genetically modified organisms in foods. Food Res Int. 2005; 38(6):639-49.
Berdal KG, Holst-Jensen A. Roundup ready soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur Food Res Technol. 2001; 213(6):432-8.
Wiseman G. State of the art and limitations of quantitative polymerase chain reaction. J AOAC Int. 2002; 85(3):792-6.
Fernandez S, Charles-Delobel C, Geldreich A, Berthier G, Boyer F, Collonnier C, et al. Quantification of the 35S promoter in DNA extracts from genetically modified organisms using real-time polymerase chain reaction and specificity assessment on various genetically modified organisms, part I: operating procedure. J AOAC Int. 2005; 88(2):547-57.
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Copyright (c) 2023 Natália Eudes Fagundes de BARROS, Edna Maria Morais OLIVEIRA, Victor Augustus MARIN
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