Influence of processing on the protein quality of new soybean cultivars intended for human food

Authors

  • Cassiano Oliveira da SILVA Universidade Federal de Viçosa
  • Gláucia Ferreira ANDRADE Universidade Federal de Viçosa
  • Maria Inês de Souza DANTAS Universidade Federal de Viçosa
  • Neuza Maria Brunoro COSTA Universidade Federal de Viçosa
  • Maria do Carmo Gouveia PELUZIO Universidade Federal de Viçosa
  • Edimar Aparecida Filomeno FONTES Universidade Federal de Viçosa
  • Hércia Stampini Duarte MARTINO Universidade Federal de Viçosa

Keywords:

Feeding, Digestibility, Food handling, Protein quality, Soybean

Abstract

Objective
The aim of this study was to evaluate the effect of processing on the protein quality of new soybean cultivars intended for human food.

Methods
The diets were prepared from the cultivars Embrapa 48, BRS 213, BRS 155 and UFVTN 105. They were heat-treated in a vacuum oven at 130ºC and 150°C, with subsequent removal or not of the hull for the production of soybean meals. A biological assay was done to determine the protein efficiency ratio, net protein ratio and true protein digestibility.

Results
Soybean meals had lower protein efficiency ratio, net protein ratio and digestibility than casein. Whole soybean meals treated at 150ºC had a better protein quality index than refined soybean meals heat-treated at 130ºC. The protein efficiency ratio and net protein ratio of Embrapa 48 and BRS 155 cultivars were higher (p<0.05) than the other ones. The UFVTN 105 cultivar had the highest digestibility (p<0.05) when treated at 130ºC. Among the cultivars heat-treated at 150ºC, the BRS 213 and BRS 155 cultivars had the lowest digestibility (p<0.05). Protein digestibility of the cultivar with low trypsin inhibitor content (BRS 155) was not better than
that of the conventional Embrapa-48 cultivar.

Conclusion
Heating and removal or not of the hull affected the protein quality of the new soybean cultivars intended for human food. Whole soybean meals heat-treated at 150ºC for 30 minutes presented the best results. Heating improved the protein quality of the conventional soybean meals. Their protein quality was similar to that of the cultivars without trypsin inhibitor and better than that of the cultivars without lipoxygenases. 

References

Magee PJ, Rowland IR. Phyto-oestrogens, their mechanism of action: current evidence for a role in breast and prostate cancer. Br J Nutr. 2004; 91: 513-31. doi: 10.1079/BJN20031075.

Zhang X, Shu XO, Li H, Yang G, Li Q, Gao Y-T, et al. Prospective cohort study of soy food consumption and risk of bone fracture among postmenopausal women. Arch Intern Med. 2005;165:1890-5.

Miura EMY, Silva RSSF, Mizubuti IY, Ida EI. Cinética de inativação de inibidores de tripsina e de insolubilização de proteínas de diferentes cultivares de soja. Rev Bras Zootec. 2005; 3:1659-65. doi: 10.1590/S1516-35982005000500028.

Monteiro MRP, Costa NMB, Oliveira MGA, Pires CV, Moreira MA. Qualidade proteica de linhagens de soja com ausência do Inibidor de Tripsina Kunitz e das isoenzimas Lipoxigenases. Rev Nutr. 2004; 17(2):195-205. doi: 10.1590/S1415-52732004000200006.

Mendes WS, Silva IJ, Fontes DO, Rodriguez NM, Marinho PC, Silva FO, et al. Composição química e valor nutritivo da soja crua e submetida a diferentes processamentos térmicos para suínos em crescimento. Arq Bras Med Vet Zootec. 2004; 56(2):207-13.

Carrão-Panizzi MC. Melhoramento genético da soja para a obtenção de cultivares mais adequados ao consumo humano. Rev Bras Nutr Clín. 2000; 15(2):330-40.

Miura EMY, Binotti MAR, Camargo DS, Mizubuti IY, Ida EI. Avaliação biológica de soja com baixas atividades de inibidores de tripsina e ausência do inibidor Kunitz. Arch Latinoam Nutr. 2001; 51(2): 1-8.

Machado FPP, Queiróz JH, Oliveira MGA, Piovesan ND, Peluzio MCG, Costa NMB, et al. Effects of heating on protein quality of soybean flour devoid of Kunitz inhibitor and lectin. Food Chem. 2008; 107:649-55. doi:10.1016/j.physletb.2003.10.071.

Silva MS, Naves MMV, Oliveira RB, Leite OSM. Composição química e valor proteico do resíduo de soja em relação ao grão de soja. Cien Tecn Aliment. 2006; 26(3):571-6. doi: 10.1590/S0101-20612006000300014.

Pires CV, Oliveira MGA, Rosa JC, Costa NMB. Qualidade e escore químico de aminoácidos de diferentes fontes proteicas. Ciênc Tecn Aliment. 2006; 26(1):179-187. doi: 10.1590/S0101-20612006000100029.

Carvalho MRB, Kirschnik PG, Paiva KC, Aiura FS. Evaluation of trypsin inhibitors activity after enzymatic digestion in heat-treated soybean. Rev Nutr. 2002; 15(3):267-72. doi: 10.1590/S1415-52732002000300002.

Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51.

Association of Official Analytical Chemists. Official Methods of Analysis of AOAC International. 17th ed. Maryland: AOAC; 1997.

Bender AE, Doell BH. Note on the determination of net protein utilization by carcass analysis. Br J Nutr. 1957; 11:138-43. doi:10.1079/BJN19570028.

Hegsted DM. Protein quality and its determination. In: Whitaker JR, Tannenbauum SR, editors. Food proteins. Westport: AVI Publishing; 1977. p.347-62.

SAS System for Windows. Version 9.00. Cary (NC): SAS Institute; 2002.

Herkelman KL, Cromwell GL, Pfeiffer TW, Knabe DA. Apparent digestibility of amino acids in raw and heated conventional and low trypsin inhibitor soybeans for pigs. J Anim Sci. 1992; 70(3): 818-26.

Friedman M, Brandon DL. Nutritional and health benefits of soy proteins. J Agric Food Chem. 2001; 49(3):1069-86. doi: 10.1021/jf0009246.

Mendes FQ, Oliveira MGA, Cardoso LR, Costa NMB, Sant’ana RCO. Digestibilidade proteica e caracterização bromatológica de linhagens de soja com ausência ou presença de inibidor de tripsina Kunitz e das isozimas lipoxigenases. Biosc J. 2007; 23(1):14-21.

Published

2023-08-28

How to Cite

Oliveira da SILVA, C. ., Ferreira ANDRADE, G., de Souza DANTAS, M. I., Maria Brunoro COSTA, N. ., Gouveia PELUZIO, M. do C., Filomeno FONTES, E. A. ., & Duarte MARTINO, H. S. (2023). Influence of processing on the protein quality of new soybean cultivars intended for human food. Brazilian Journal of Nutrition, 23(3). Retrieved from https://periodicos.puc-campinas.edu.br/nutricao/article/view/9375

Issue

Section

ORIGINAL ARTICLE