Effects of a whey protein concentrate and it’s peptides in the protection of ulcerative lesions at rat gastric mucosa
Keywords:
protein hydrolysates, peptides, rats, Wistar, stomach ulcerAbstract
Objective
To assess the ability of bovine whey protein hydrolysate and its low molecular weight fraction (molecular
weight ≤1kDa) to protect the gastric mucosa of rats against ulcerative process induced by three different
agents.
Methods
Adult Wistar rats were subjected to the indomethacin-induced ulcer (30mg/kg body weigh), absolute ethanol
(1ml/animal) and immobilization and cold stress (40 C/2h), models.
Results
Whey protein hydrolysate was obtained by treatment with pancreatin to a degree of hydrolysis of 20% and
fractionated using a tangential flow membrane with a molecular weight cut-off of 1kDa to obtain the fraction
containing low molecular weight peptides (≤1kDa). In the ethanol-induced acute ulcer model (single dose),
whey protein hydrolysate inhibited the gastric lesion index by 65.5% and the double dose resulted in a 77.4%
inhibition.
Conclusion
For the anti-inflammatory model, the cytoprotective effect of low molecular weight peptides was stronger
than that of total hydrolysate (53.1 and 71.6%, ulcerative lesion index) for single and double dose, respectively.
No mucosa cytoprotective activity was found for whey protein concentrate, whey protein hydrolysate or WPHP
in the immobilization and cold stress model.
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Copyright (c) 2023 Maria Teresa Bertoldo PACHECO, Érica BIGHETTI, Márcia ANTÔNIO, João Ernesto de CARVALHO, Caroline Filla ROSANELI, Valdemiro Carlos SGARBIERI
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