Influence of whey protein on liver enzymes, lipid profile and bone formation of hypercholesterolemic rats
Keywords:
Amino Acids, Aspartate aminotransferases, Alkaline phosphatase, Cholesterol, Hypercholesterolemia, Peptides and proteinsAbstract
Objective
The aim of this study was to assess the effect of whey protein consumption on the lipid, hepatic and renal metabolism, bone formation and antioxidant defense system of rats given a hypercholesterolemic diet.
Methods
A total of 32 adult Fisher rats were divided into 4 groups: C group, standard diet; H group, hypercholesterolemic diet; WP group, standard diet with whey protein; WPH group, hypercholesterolemic diet with whey protein. Food ingestion and weight gain were monitored weekly. After 8 weeks, blood was collected for biochemical analysis and the rats were killed. The statistical tests analysis of variance and Tukey’s test were performed.
Results
Whey protein did not lower serum cholesterol significantly and increased serum triglycerides in hypercholesterolemic rats. However, whey protein decreased the activity of aspartate aminotransferase and alkaline phosphatase, as well as serum creatinine. The assessed antioxidant defenses were not affected. Diets containing whey protein generated heavier, thicker and longer bones than casein diets.
Conclusion
Whey proteins did not have a significant hypocholesterolemic effect in rats. On the other hand, they significantly prevented changes in liver and kidney functions brought about by hypercholesterolemic diets. Data also suggest that whey proteins benefit bone formation when compared with casein diets.
References
Sgarbieri VC. Propriedades fisiológicas-funcionais das proteínas do soro do leite. Rev Nutr. 2004; 17(4):397-409.
Lönnerdal B. Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr. 2003; 77(6):1537s-43s.
De Wit JN. Nutritional and functional characteristics if whey proteins in foods products. J Dairy Sci. 1998; 81(3):597-608.
Ha E, Zemel MB. Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people. J Nutr Biol. 2003; 14(5):251-8.
Kinsella JE, Whitehead DM. Proteins in whey: chemical, physical and functional properties. Adv Foods Nutr Res. 1989; 33(1):343-438.
Haraguchi FK, Abreu WC, De Paula H. Proteínas do soro: composição, propriedades nutricionais, aplicações no esporte e benefícios para a saúde humana. Rev Nutr. 2006; 19(4):479-88.
Lands LC, Grey VL, Smoutas AA. Effect of supplementation with cysteine donor on muscular performance. J Appl Physiol. 1999; 87(4):1381-5.
McIntosh GH, Le Leu RK. The influence of dietary proteins on colon cancer risk. Nutr Res. 2001; 21(7): 1053-66.
Micke P, Beeh KM, Buhl R. Effects of long-term supplementation with whey proteins on plasma glutathione levels of HIV-infected patients. Eur J Nutr. 2002; 41(1):12-8.
Nagaoka S, Kanamuru Y, Kuzuya Y, Kojima T,Tomotsu K. Comparative studies on the serum cholesterol lowering action of whey protein and soybean protein in rats. Biosci Biotechnol Biochem. 1992; 56(9):1484-5.
Sautier C, Dieng K, Flament C, Doucet C, Suquet JP, Lemonnier D. Effects of whey protein, casein, soya-bean and sunflower proteins on the serum, tissue and faecal steroids in rats. Br J Nutr. 1983; 49(3):13-9.
Choi YS, Ikeda I, Sugano M. The combined effects of dietary proteins and fish oil on cholesterol metabolism in rats of different ages. Lipids. 1989; 24(6):506-10.
Zhang X, Beynen A. Lowering effect of dietary milkwhey protein v. casein on plasma and liver cholesterol concentrations in rats. Br J Nutr. 1993; 70(1):139-46.
Jacobucci HB, Dias NGP, Sgarbieri VC, Borges PZ, Tanikawa C. Impact of different dietary protein on rat growth, blood serum lipids and protein, and liver cholesterol. Nutr Res. 2001; 21(6):905-15.
Takada Y, Aoe S, Kumegawa M. Whey protein stimulated the proliferation and differentiation of osteoblastic MC3T3-E1 cells. Biochem Biophys Res Commun. 1996; 223(2):445-9.
Toba Y, Takada Y, Yamamura J, Tanaka M, Matsuoka Y, Kawakami H, et al. Milk basic protein: a novel protective function of milk against osteoporosis. Bone. 2000; 27(3):403-8.
Marshall K. Therapeutic applications of whey protein. Altern Med Rev. 2004; 9(2):136-56.
Yamamura J, Aoe S, Toba Y, Motouri M, Kawakami H, Kumegawa M, et al. Milk basic protein (MBP) increases radial bone mineral density in healthy adult women. Biosci Biotechnol Biochem. 2002; 66(3):702-4.
Aoe S, Toba Y, Yamamura J, Kawakami H, Yahiro M, Kumegawa M, et al. Controlled trial of the effects of milk basic protein (MBP) supplementation on bone metabolism in healthy adult women. Biosci Biotechnol Biochem. 2001; 65(4):913-8.
Reeves FG, Nielsen FH, Fahey Jr JC. AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. J Nutr. 1993; 123(11):1939-51.
Association of Official Analytical Chemists. Official methods of analysis of the AOAC. 15th ed. Arlington: AOAC;1990.
Lee SC, Prosky L, Devries JW. Determination of total, soluble and insoluble dietary fiber in foods. Enzimatic-gravimetric method, MES-TRIS buffer: collaborative study. J Assoc Off Chem Int. 1992; 75:395-416.
Beltowski J, Wójcicka G, Jamroz A. Leptin decreases plasma paraoxonase 1 (PON 1) activity and induces oxidative stress: the possible novel mechanism for proatherogenic effect of chronic hyperleptinemia. Atherosclerosis. 2003; 170(1):21-9.
Sedlak J, Lindsay RH. Estimation of total, proteinbound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968; 25(1): 192-205.
Lovati, MR, West CE, Sirtori CR, Beynen AC. Dietary animal proteins and cholesterol metabolism in rabbits. Br J Nutr. 1990; 64(2):473-85.
Aviram M. Introduction to the serial review on paraoxonases, oxidative stress, and cardiovascular diseases. Free Radical Biol Med. 2004; 37(9): 1301-3.
Shih DM, Gu L, Hama S, Xia YR, Navab M, Fogelman AM, et al. Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model. J Clin Investig. 1996; 97(7): 1630-9.
Ozaki M, Fuchinoue S, Teraoda S, Ota K. The in vivo cytoprotection of ascorbic acid against ischemia/reoxygenation injury of rat liver. Arch Biochem Biophys. 1995; 318(2):439-45.
Kanhal MA, Ahmad F, Othman AA, Arif Z, Orf S, Murshed KS. Effect of pure and oxidized cholesterol-rich diet on some biochemical parameters in rats. Int J Food Sci Nutr. 2002; 53(5): 381-8.
Fukatsu T. Alkaline phosphatase. Rinsho Byori. 2001; Suppl 116:27-35.
Muriel P. High fat diet and liver damage induced by biliary obstruction in the rat. J Appl Toxicol. 1995; 15(2):125-8.
Souza DM, Rocha D, Kantorski KZ, Rocha RF. Influência do consumo crônico de álcool etílico na morfologia de ossos longos em ratas adultas jovens. Ciênc Odontol Bras. 2007; 10(1):19-25.
Toba Y, Takada Y, Itabashi A, Morita Y, Hirai T, Suguri T, et al. Milk basic protein promotes bone formation and suppresses bone resorption in healthy adult men. Biosci Biotechnol Biochem. 2001; 65(6):1353-7
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Copyright (c) 2023 Fabiano Kenji HARAGUCHI, Maria Lucia PEDROSA, Heberth de PAULA, Rinaldo Cardoso dos SANTOS, Marcelo Eustáquio SILVA
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