Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics
Keywords:
Inulin, Klebsiella sp, Enteral nutrition, Bacterial translocationAbstract
Objective
This work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice.
Methods
Four- to six-week–old Swiss albino mice were divided into nine groups and fed enteral diets containing different combinations of fructooligosaccharide, inulin, antibiotic and corticoid, inoculated or not with Klebsiella pneumoniae. On day 5, the animals of four groups were fed an enteral diet contaminated with approximately 1010CFU/g of K. pneumoniae. At defined times, two animals of each group were sacrificed and their organs (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies.
Results
A higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet.
Conclusion
The addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug.
References
Gales AC, Sader HS, Jones RN. Respiratory tract pathogens isolated from patients hospitalized with suspected pneumonia in Latin America: frequency of occurrence and antimicrobial susceptibility profile. Results from SENTRY Antimicrobial Surveillance Program (1997-2000). Diag Microbiol Infect Dis. 2002; 44(3):301-11.
Mehall JR, Kite CA, Saltzman DA, Wallett T, Jackson RJ, Smith SD. Prospective study of incidence and complications of bacterial contamination of enteral feeding in neonates. J Ped Surg. 2002; 37(8): 1177-82.
Oliviera MH, Bonelli R, Aidoo KE, Batista RV. Microbiological quality of reconstituted enteral formulations used in hospitals. Nutrition. 2000; 16(9):729-33.
Pinto UM, Cardoso RR, Vanetti MCD. Detecção de Listeria, Salmonella e Klebsiella em serviço de alimentação hospitalar. Rev Nutr. 2004; 17(3): 319-26.
Berg RD. Bacterial translocation from the gastrointestinal tracts of mice receiving immunosuppressive chemotherapeutic agents. Curr Microbiol. 1983; 8(5):879-82.
Wiest R, Rath HC. Bacterial translocation in the gut. Best Prac Res Clin Gastroenterol. 2003; 17(3): 397-425.
Kapkac M, Erikoglu M, Tuncyurek P, Ersin S, Esassolak M, Alkanal M, et al. Fiber enriched diets and radiation induced injury of the gut. Nutr Res. 2003; 23(1):77-83.
Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microflora: introducing of prebiotics. J Nutr. 1995; 125(6):1401-12.
Kaplan H, Hutkis RW. Fermentation of fructooligosaccharides by lactic acid bacteria and bifidobacteria. Appl Environ Microbiol. 2000; 66(6): 2682-4.
Guarner F, Malagelada JR. Gut flora in health and disease. Lancet. 2003; 361(9356):512-9.
Rayes N, Hansen S, Seehofer D, Müller AR, Serke S, Bengmark S, et al. Early enteral supply of fiber and lactobacilli versus conventional nutrition: a controlled trial in patients with major abdominal surgery. Nutrition. 2002; 18(7/8):609-15.
Pereira SCL. Caracterização molecular e de fatores de virulência de Klebsiella sp. isoladas de dietas enterais [tese]. Viçosa: Universidade Federal de Viçosa; 2001.
Reeves PG, Nielsen RH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51.
Nader de Macíaz ME, Apella MC, Romero NC, González SN, Oliver G. Inhibition of Shigella sonnei by Lactobacillus casei and Lactobacillus acidophilus. J Appl Bacteriol. 1992; 73(5):407-11.
Berg RD. Bacterial translocation from the gastrointestinal tract. Trends Microbiol. 1995; 3(4):149-54.
Bagley ST, Seidler RJ. Primary Klebsiella identification with MacConkey-Inositol-Carbenicillin agar. Appl Environ Microbiol. 1978; 36(3):536-8.
Gianotti L, Alexander JW, Fukushima R, Pyles T. Steroid therapy can modulate gut barrier function, host defense, and survival in thermally injured mice. J Surg Res. 1996; 62(1):53-8.
Alverdy J, Aoys E. The effect of glucocorticoid administration on bacterial translocation. Am Surg. 1991; 214(6):719-23.
Jones WG, Barber AE, Kapur S, Hawes AJ, Fahey TJ, Minei JP, et al. Pathophysiologic glucocorticoid levels and survival of translocating bacteria. Arch Surg. 1991; 126(1):50-5.
Roberfroid MB, van Loo JAE, Gibson GR. The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr. 1998; 128(1):11-9.
Manning TS, Gibson GR. Prebiotics. Best Prac Res Clin Gastroenterol. 2004; 18(2):287-98.
Roberfroid, M. Functional food concept and its application to prebiotics. Dig Liver Dis. 2002; 34(2): S105-S10.
Wile TV, Boon N, Possemiers S, Jacobs H, Verstraete W. Prebiotic effects of chicory inulin in the simulator of the human intestinal microbial ecosystem. FEMS Microbiol Ecol. 2004; 51(1): 143-53.
Montesi A, Garcia-Albiach RG, Pozuelo MJ, Pintado C, Goni I, Rotger R. Molecular and microbiological analysis of caecal microbiota in rats fed with diets supplemented either with prebiotics or probiotics. Int J Food Microbiol. 2005; 98(3):281-9.
Rinne M, Gueimonde M, Kalliomäki M, Hoppu U, Salminen SJ, Isolauri E. Similar bifidogenic effects of prebiotic-supplemented partially hydrolyzed infant diet and breastfeeding on infant gut microbiota. FEMS Immunol Med Microbiol. 2005; 43(1):59-65.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Daniele Ferreira da SILVA, José Mário da Silveira MEZÊNCIO, Maria Cristina Dantas VANETTI, Paulo Roberto CECON, Matheus Loureiro dos SANTOS
This work is licensed under a Creative Commons Attribution 4.0 International License.
