Suplementação com ácido linoléico conjugado: estabilidade oxidativa dos suplementos e correlações com conteúdo dos lípides totais hepáticos e indicadores da oxidação dos lípides biológicos de ratos Wistar
Palabras clave:
Ácidos linoléicos conjugados, Estresse oxidativo, LípidesResumen
Objetivo
O objetivo do trabalho foi avaliar a estabilidade oxidativa de misturas comerciais de ácido linoléico conjugado e buscar possível correlação entre a suplementação e o conteúdo total de lípides hepáticos, e também de alguns indicadores da oxidação lipídica em ratos.
Métodos
Um ensaio biológico com 30 ratos divididos em três grupos (n=10) caracterizando os grupos controle e suplementados com as misturas comerciais AdvantEdge® e One® foi realizado. A concentração administrada foi de 2% em relação ao consumo de dieta e os animais foram suplementados durante 42 dias. O conteúdo total de lípides do fígado foi determinado e a morfologia do órgão foi examinada por meio de microscopia ótica. Índice de peróxido e malondialdeído foram determinados para avaliar a estabilidade oxidativa dos suplementos in vitro. Índice de peróxido, malondialdeído, 8-iso-PGF2D isoprostana e catalase foram determinados como indicadores da oxidação dos lípides biológicos.
Resultados
Os resultados demonstraram baixa estabilidade das misturas comerciais à oxidação in vitro. As associações entre o consumo de ácido linoléico conjugado e malondialdeído (r=-0,7914, p<0,0001) e catalase (r=-0,5991, p=0,008) foram moderadas, negativas e significantes, demonstrando que o ácido linoléico conjugado reduziu a oxidação lipídica in vivo. O conteúdo dos lípides totais hepáticos não aumentou (22,42%, DP=1,40%) e a morfologia do órgão permaneceu íntegra.
Conclusão
Embora esse protocolo de suplementação com ácido linoléico conjugado tenha reduzido a oxidação lipídica, há que considerar a tendência para o aumento de 8-iso-PGF2D isoprostana e de peróxidos como perspectiva de continuidade das pesquisas sobre a reinvestigação do efeito antioxidante do ácido linoléico conjugado.
Citas
Van Den Berg JJM, Cook NE, Tribble DL. Reinvestigation of the antioxidant properties of conjugated linoleic acid. Lipids. 1995; 30(5)599-605.
Cantwell H, Devery R, Stanton C, Lawless F. The effect of a conjugated linoleic acid on superoxide dismutase, catalase and glutathione peroxidase in oxidatively-challenged liver cells. Biochem Soc T. 1998; 26(1):52.
Banni S, Angioni E, Contini MS, Carta G, Casu V, Iengo GA, et al. Conjugated linoleic acid and oxidative stress. J Am Oil Chem Soc. 1998; 75(2):261-67.
Cantwell H, Devery R, O’shea M, Stanton C. The effect of conjugated linoleic acid on the antioxidant enzyme defense system in rat hepatocytes. Lipids. 1999; 34(8):833-39.
Yamasaki M, Mansho K, Mishima H, Kimura G, Sasaki M, Kasai M, et al. Effect of dietary conjugated linoleic acid on lipid peroxidation and histological change in rat liver tissues. J Agr Food Chem. 2000; 48(12):6367-71.
Basu S, Risérus U, Turpein A, Vessby B. Conjugated linoleic acid induces lipid peroxidation in men with abdominal obesity. Clin Sci. 2000; 99(6):511-16.
Basu S, Smedman A, Vessby B. Conjugated linoleic acid induces lipid peroxidation in humans. FEBS Letters. 2000; 468(1):33-36.
Risérus U, Basu S, Jovinge S, Fredrikson GN, Ärnlöv J, Vessby B. Supplementation with conjugated linoleic acid causes isomer-dependent oxidative stress and elevated C-reactive protein. Circulation. 2002; 106(15):1925-29.
Risérus U, Vessby B, Ärnlöv J, Basu S. Effects of cis-9, trans-11 conjugated linoleic acid supplementation on insulin sensitivity, lipid peroxidation, and proinflammatory markers in obese men. Am J Clin Nutr. 2004; 80(2):279-83.
Smedman A, Vessby B, Basu S. Isomer-specific effects of conjugated linoleic acid on lipid peroxidation in humans: regulation by D-tocopherol and cyclo-oxygenase-2 inhibitor. Clin Sci. 2004; 106(1):67-73.
Kim JH, Hubbard NE, Ziboh V, Erickson KL. Attenuation of breast tumor cell growth by conjugated linoleic acid via inhibition of 5-lipoxygenase activating protein. Biochim Biophys Acta. 2005; 1736(3):244-50.
Arab K, Rossary A, Soulère L, Steghens JP. Conjugated linoleic acid, unlike other unsaturated fatty acids, strongly induces glutathione synthesis without any lipoperoxidation. Brit J Nutr. 2006; 96(5):811-19.
Belury MA, Kempa-Steczko A. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids. 1997; 32(2):199-204.
Cherian G, Holsonbake TB, Goeger MP, Bildfell R. Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. Lipids 2002; 37(8):751-7.
Association of Official Analytical Chemists. Official methods of analysis. 16th ed. Virgínia: AOAC International; 1995. v.1.
Sinnhuber RO, Yu TC. 2-Thiobarbituric acid method for the measurement of rancidity in fishery products. II. The quantitative determination of malonaldehyde. Food Technol. 1958; 12(1):9-12.
Reeves PG, Nielsen FH, Fahey GCJ. AIN-93 Purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc Writing Committee on the Reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51.
Goena M, Mazo F, Fernández-González L, Tosar A, Fruhbeck G, Santidrián S. Effect of the raw legume Vicia ervilha on muscle and liver protein metabolism in growing rats. Rev Española Fisiol. 1989; 45:55-60.
Bligh EG, Dyer WJ. A rapid method of total lipid extration and purification. Can J Biochem Physiol. 1959; 37(8):911-7.
Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991; 196(2-3):143-52.
Behmer OA, Tolosa EMC, Neto AGF. Manual de técnicas para histologia normal e patológica. São Paulo: Edart Editora; 1976.
SAS Institute Project for Windows: user’s guide statistics. Version 8.0. Cary (USA); 2003.
Yang L, Leung LK, Huang Y, Chen ZY. Oxidative stability of conjugated linoleic acid isomers. J Agric Food Chem. 2000; 48(8):3072-76.
Tsuzuki T, Igarashi M, Iwata T, Ymauchi-Sato Y, Yamamoto T, Ogita K, et al. Oxidation rate of conjugated linoleic acid and conjugated linolenic acid is slowed by triacylglycerol esterification and a-tocopherol. Lipids. 2004; 39(5):475-80.
Miyazawa T, Tsuzuki T, Nakagawa K, Igarashi M. Fatty acids with conjugated unsaturation: relationship between oxidative stability and physiological activities. Lipid Technol. 2005; 17(10): 221-5.
Ha YL, Storkson J, Pariza M. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated linoleic acid. Cancer Res. 1990; 50(4):1097-101.
Flintoff-Dye NL, Omaye ST. Antioxidant effects of conjugated linoleic acid isomers in isolated human low-density lipoproteins. Nutr Res. 2005; 25(4): 1-12.
Roberts LJ, Morrow JD. Measurement of F2-isoprostanes as an index of oxidative stress in vivo. Free Radical Bio Med 2000; 28(1):505-13.
Cherian G, Goeger MP. Hepatic lipid characteristics and histopathology of laying hens fed CLA or n-3 fatty acids. Lipids. 2004; 39(4):31-6.
Macarulla MT, Fernández-Quintela A, Zabala A, Navarro V, Echevarría E, Churruca I, et al. Effects of conjugated linoleic acid on liver composition and fatty acid oxidation are isomer-dependent in hamster. Nutrition. 2005; 21(1):512-9.
Moya-Camarena SY, Vanden Heuvel JP, Blanchard SG, Leesnitzer LA, Belury MA. Conjugated linoleic acid is a potent naturally occurring ligand and activator of PPARa. J Lipid Res. 1999; 40(4): 1426-33.
Choi YJ, Kim YC, Han YB, Park Y, Pariza MW, Ntambi JM. The trans-10, cis-12 isomer of conjugated linoleic acid downregulated stearoyl-CoA desaturase gene expression in 3T3-L1 adipocytes. J Nutr. 2000; 130(8):1920-24.
Belury MA, Moya-Camarena SY, Lu M, Shi L, Leesnitzer LM, Blanchard SG. Conjugated linoleic acid is na activator and ligand for peroxissome proliferator-activated receptor-gama (PPARg). Nutr Res. 2002; 22(7):817-24.
Kang K, Liu W, Albright KJ, Park Y, Pariza MW. Trans10, cis-12 inhibits differentiation of 3T3-L1 adipocytes and decrases PPAR gamma expression. Biochem Bioph Res Co. 2003; 303(3):795-9.
Cimini AM, Cristiano L, Colafarina S, Benedetti E, Di Loreto S, Festuccia C, et al. PPARg-dependent effects of conjugated linoleic acido in the human glioblastoma cell line (ADF). Int J Cancer. 2005; 117(6):923-33.
Bassaganya-Riera J, Hontecillas R. CLA and n-3 PUFA differentially modulate clinical activity and colonic PPAR-responsive gene expression in a pig model of experimental IBD. Clin Nutr. 2006; 25(3): 454-65.
Ringseis R, Müller A, Herter C, Gahler S, Steinhart H, Eder K. CLA isomers inhibit TNFa-induced eicosanoid release from human vascular smooth muscle cells via a PPARg ligand-like action. Biochim Bioph Acta. 2006; 1760(2):290-300.
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Derechos de autor 2023 Lilia Ferreira SANTOS-ZAGO, Adriana Prais BOTELHO, Admar Costa de OLIVEIRA (in memoriam)
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
