Polyunsaturated fatty acids omega-3 supplementation reduces inflammatory markers andimproves insulin action in liver of mice
Keywords:
Fatty acids, insaturated, Liver, Inflammation, Insulin resistanceAbstract
Objective
The aim of the present study was to assess the effects of omega-3 polyunsaturated fatty acid supplementation on insulin signaling and the pro-inflammatory pathway in the liver tissue of mice.
Methods
Swiss mice were divided into six groups given different dosages of fish oil containing omega-3 (1mg, 5mg, 10mg and 50mg) by gavage. The control group was given water. Fasting plasma glucose was measured on days 0 (no supplementation), 14 and 21 (after supplementation) to determine the dosage and time-dependent effects of omega-3. Because the group n-3-21days (21 days of omega-3 supplementation) demonstrated lower blood glucose, this time interval was selected for molecular analysis. After an 8-hour fast, liver tissue samples were taken from the control and n-3-21days groups, and western blot analyses were performed to
assess insulin signaling and the pro-inflammatory pathway.
Results
The results showed that the dosage of 10mg leads to greater reduction in blood glucose at 14 and 21 days when compared with other dosages. Thus, this dosage was selected for molecular analysis. This dosage significantly decreased phosphorylation of c-Jun N-terminal protein kinase and Ik B kinase and protein levels of nuclear factor Kappa-light-chain-enhancer of activated B cells. In parallel, increased insulin pathway signaling was observed, as confirmed by increases in insulin receptors, insulin receptor substrate 1 and protein kinase B phosphorylation.
Conclusion
The present study suggests that omega-3 fatty acids improve insulin signaling by reducing inflammation. These results may be one of the explanations for low fasting glucose.
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Copyright (c) 2023 Gabrielle da LUZ, Sabrina da SILVA, Schérolin MARQUES, Thaís Fernandes LUCIANO, Cláudio Teodoro de SOUZA
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