Capacidade antioxidante total de óleos vegetais comestíveis
determinantes químicos e sua relação com a qualidade dos óleos
Palabras clave:
Antioxidantes, Óleos vegetais, Peroxidação de lipídeos, Radicais livresResumen
A capacidade antioxidante total de óleos vegetais comestíveis é determinada por sua composição físico-química e pode estar associada a atributos de qualidade dos óleos, especialmente a sua bioatividade e possivelmente a sua estabilidade oxidativa. Este artigo apresenta os fundamentos dos ensaios de capacidade antioxidante total e avalia criticamente os ensaios aplicáveis na análise de óleos e os pontos críticos nas aplicações dos ensaios para a análise dessas amostras. Discute-se o potencial papel dos componentes químicos dos óleos comestíveis como determinantes da capacidade antioxidante total, assim como a possível relação da capacidade antioxidante com a bioatividade e a estabilidade oxidativa dos óleos. Finalmente, discutem-se evidências de que, caso seja sistematicamente investigado em trabalhos experimentais futuros, o uso de ensaios de capacidade antioxidante total na análise de óleos vegetais pode contribuir para integrar o conhecimento da composição química com a bioatividade e possivelmente com a estabilidade de óleos vegetais específicos. Dessa forma, os ensaios de capacidade antioxidante apresentam potencial para aplicação no controle da qualidade integral de óleos comestíveis.
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Szyd owska-Czerniak A, Karlovits G, Dianoczki C, Recseg K, SzB yk E. Comparison of two analytical methods for assessing antioxidant capacity of rapeseed and olive oils. J Am Oil Chem Soc. 2008; 85:141-49. doi: 10.1007/s11746-007-1178-6.
Ramadan MF, Moersel JT. Screening of the antiradical action of vegetable oils. J Food Compost Anal. 2006; 19:838-42. doi: 10.1016/j.jfca.2006.0 2.013.
Chaiyasit W, Elias RJ, McClements DJ, Decker EA. Role of physical structures in bulk oils on lipid oxidation. Crit Rev Food Sci Nutr. 2007; 47:299-317. doi: 10.1080/10408390600754248.
Wu X, Gu L, Holden J, Haytowitz DB,.Gebhardt SE, Beecher G, et al. Development of a database for total antioxidant capacity in foods: a preliminary study. J Food Compost Anal. 2004; 17:407-22. doi: 10.1016/j.jfca.2004.03.001.
Huang D, Ou B, Prior R. The Chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005; 53:1841-56. doi: 10.1021/jf030723c.
Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005; 53:4290-302. doi: 10.1021/jf05 02698.
Ninfali P, Aluigi G, Bacchiocca M, Magnani M. Antioxidant capacity of extra-virgin olive oils. J Am Oil Chem Soc. 78:243-7. doi: 10.1007/s11746-0 01-0252-9.
Magalhães LM, Segundo MA, Reis S, Lima JLFC. Methodological aspects about in vitro evaluation of antioxidant properties. Anal Chim Acta. 2008; 613:1-19. doi: 10.1016/j.aca.2008.02.047.
Pérez-Jiménez J, Arranz S, Tabernero M, Díaz-Rubio ME, Serrano J, Goñi I, et al. Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: extraction, measurement and expression of results. Food Res Intern. 2008; 41: 274-85. doi: 10.1016/j.foodres.2007.12.004.
Pérez-Jiménez J, Saura-Calixto F. Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res Int. 2006; 39:791-800. doi: 10.1016/j.foodres.2006.02.003.
Sherwin ER. Antioxidants for vegetable oils. J Am Oil Chem Soc. 1976; 53:430-6. doi: 10.1007/BF026 05739.
Frankel EN, Huang SW, Kanner J, German JB. Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. J Agric Food Chem. 1994; 42:1054-9. doi: 10.1021/jf00041 a001.
Frankel EN. Antioxidants in lipid foods and their impact on food quality. Food Chem. 1996; 57:51-5. doi: 10.1016/0308-8146(96)00067-2.
Espín JC, Soler-Rivas C, Wichers HJ. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl1-picrylhydrazyl radical. J Agric Food Chem. 2000; 48:648-56. doi: 10.1021/jf9908188.
Tuberoso CIG, Kowalczyk A, Sarritzu E, Cabras P. Determination of antioxidant compounds and antioxidant activity in commercial oilseeds for food use. Food Chem. 2007; 103:1494-501. doi: 10.10 16/j.foodchem.2006.08.014.
Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, et al. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J Nutr. 2003; 133:2812-19.
SzydB owska-Czerniak A, Dianoczki C, Recseg K, Karlovits G, SzB yka E. Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta. 2008; 76:899-905. doi: 10.1016/j.talanta.2008.04.055.
Miraliakbari H, Shahidi F. Antioxidant activity of minor components of tree nut oils. Food Chem. 2008; 111:421-27. doi: 10.1016/j.foodchem. 200 8.04.008.
Jimenez-Alvarez D, Giuffrida F, Vanrobaeys F, Golay PA, Cotting C, Lardeau A, et al . High-throughput methods to assess lipophilic and hydrophilic antioxidant capacity of food extracts in vitro. J Agric Food Chem. 2008; 56:3470-77. doi: 10.1021/jf70 3723s.
Valavanidis A, Nisiotou C, Papageorgiou Y, Kremli I, Satravelas N, Zinieris N, et al. Comparison of the radical scavenging potential of polar and lipidic fractions of olive oil and other vegetable oils under normal conditions and after thermal treatment. J Agric Food Chem. 2004; 52:2358-65. doi: 10.10 21/jf030491h.
Lee JM, Chung H, Chang OS, Lee JH. Development of a method predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chem. 2006; 103(2):662-9. doi: 10.10 16/j.foodchem.2006.07.052
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9/10):1231-7. doi: 10.1016/S0891-5849(98)00315-3.
Sanchez CS, González AMT, García-Parrilla MC, Granados JJQ, La Serrana HLG, Martinez MCL. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal Chim Acta. 2007; 593:103-7. doi: 10.1016/j.aca.20 07.04.037.
Gorinstein S, Martin-Belloso O, Katrich E, Lojek A, Ciz M, Gligelmo-Miguel N, et al. Comparison of the contents of the main biochemical compounds and the antioxidant activity of some Spanish olive oils as determined by four different radical scavenging tests. J Nutr Biochem. 2003; 14:154-59. doi: 10.1016/S0955-2863(02)00278-4.
Ninfali P, Bacchiocca M, Biagiotti M, Servili M, Montedoro G. Validation of the oxygen radical absorbance capacity (ORAC) parameter as a new index of quality and stability of virgin olive oil. J Am Oil Chem Soc. 2002; 79:977-82. doi: 10.1007/s 11746-002-0590-7.
Huang D, Ou B, Hmpsch-Woodill M, Flanagan JA, Deemer EK. Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated βcyclodextrin as the solubility enhancer. J Agric Food Chem. 2002; 50:1815-21. doi: 10.1021/jf01137 32.
Saldeen K, Saldeen T. Importance of tocopherols beyond α-tocopherol: evidence from animal and human studies. Nutr Res. 2005; 25:877-89. doi: 10.1016/j.nutres.2005.09.019.
Rossi M, Almprese C, Ratti S. Tocopherols and tocotrienols as free radical-scavengers in refined vegetable oils and their stability during deep-fat frying. Food Chem. 2007; 102:812-17. doi: 10.10 16/j.foodchem.2006.06.016.
Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial byproducts: antioxidant activity, occurrence, and potential uses. Food Chem. 2006; 99:191-203. doi: 10.1016/j.foodchem.2005.07.042.
Gutfinger T. Polyphenols in olive oils. J Am Oil Chem Soc. 1981; 58(11):966-68. doi: 10.1007/BF0265 9771
Del Carlo M, Sacchetti G, Di Mattia C, Compagnone D, Mastrocola D, Liberatore L, et al. Contribution of the phenolic fraction to the antioxidant activity and oxidative stability of olive oil. J Agric Food Chem. 2004; 52:4072-79. doi: 10.1021/jf049806z.
Carrasco-Pancorbo A, Cerretani L, Bendini A, Segura-Carretero A, Del Carlo M, Gallina-Toschi, et al. Evaluation of the antioxidant capacity of individual phenolic compounds in virgin olive oil. J Agric Food Chem. 2005; 53:8918-25. doi: 10.10 21/jf0515680.
Dabbou S, Brahmi F, Taamali A, Issaoui M, Ouni Y, Braham M, et al. Extra virgin olive oil components and oxidative stability from olive oils grown in Tunisia. J Am Oil Chem Soc. doi: 10.1007/s11746-0 10-1600-3.
Choe E, Min DB. Mechanisms and factors for edible oil oxidation. Compr Rev Food Sci Food Saf. 2006; 5:169-86. doi: 10.1111/j.1541-4337.2006.0000 9.x.
Huang SW, Frankel EN, German JB. Antioxidant activity of α- and γ- tocopherols in bulk oils and in oil water emulsions. J Agric Food Chem. 1994; 42: 2108-14. doi: 10.1021/jf00046a007.
Huang SW, Frankel EN, German JB. Effects of individual tocopherols and tocopherol mixtures on the oxidative stability of corn oil triglycerides. J Agric Food Chem. 1995; 43:2345-50. doi: 10.1021/jf0 0057a006.
Mkinen M, Kamal-Eldin A, Lampi AMA, Hopia A. Effects of α- and γ-tocopherols on formation of hydroperoxides and two decomposition products from methyl linoleate. J Am Oil Chem Soc. 2000; 77:801-06. doi: 10.1007/s11746-000-0128-z
Bendini A, Cerretani L, Carrasco-Pancorbo A, Gómez-Caravaca AM, Segura-Carretero A, et al. Phenolic molecules in virgin olive oils: a survey of their sensory properties, health effects, antioxidant activity and analytical methods: an overview of the last decade. Molecules. 2007; 12:1679-719. doi: 10.3390/12081679.
Aparício R, Roda L, Albi MA, Gutiérrez F. Effect of various compounds on virgin olive oil stability measured by Rancimat. J Agric Food Chem. 1999; 47,4150-5. doi: 10.1021/jf9812230.
Psomiadou E, Tsimidou M. Stability of virgin olive oil. 1. Autoxidation studies. J Agric Food Chem. 2002; 50:716- 21. doi: 10.1021/jf0108462.
Halliwell, B. Antioxidants in human health and disease. Annu Rev Nutr. 1996; 16:33-50. doi: 10.11 46/annurev.nu.16.070196.000341.
Pellegrini N, Salvatore S, Valtuen S, Bedogni G, Porrini M, Pala V, et al. Development and validation of a food frequency questionnaire for the assessment of dietary total antioxidant capacity. J Nutr. 2007; 137:93-8.
Covas MI, Ruiz-Gutiérrez V, de la Torre R, Kafatos A, Lamuela-Raventós RM, Osada J, et al. Minor components of olive oil: evidence to date of health benefits in humans. Nutr Rev. 2006; 64:S20-S30. doi: 10.1111/j.1753-4887.2006.tb00260.x.
Castelo-Branco VN, Torres AG. Potential application of antioxidant capacity assays to assess the quality of edible vegetable oils. Lipid Technology. 2009; 21(7):152-5. doi: 10.1002/lite.200900035.
Holst B, Williamson G. Nutrients and phytochemicals: from bioavailability to bioefficacy beyond antioxidants. Curr Opin Biotechnol. 2008; 19:73-82. doi: 10.1016/j.copbio.2008.03.003.
Saura-Calixto F, Goni I. Antioxidant capacity of the Spanish Mediterranean diet. Food Chem. 2006; 94:442-7. doi: 10.1016/j.foodchem.2004.11.033.
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Derechos de autor 2023 Vanessa Naciuk CASTELO-BRANCO, Alexandre Guedes TORRES
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
