Effect of varied doses of retinoic acid on young rats’ bone resistance

Authors

  • Luciana Bronzi de SOUZA Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Departamento de Saúde Pública.
  • Cristiana Maria Murbach FREIRE Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Departamento de Clínica Médica
  • Renata Noêmia Alves de ALMEIDA Nutricionista.
  • Sérgio Swain MÜLLER Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Departamento de Cirurgia e Ortopedia.
  • Sérgio Alberto Rupp PAIVA
  • Gláucia Maria Ferreira da Silva MAZETO Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Departamento de Clínica Médica.

Keywords:

Biomechanics, Osteoporosis, Rats, Tretinoin

Abstract

Objective
This study assessed the effects of different doses of all-trans retinoic acid on bone resistance by conducting a biomechanical flexion study on young rats’ tibias.
Methods
Fifty-eight young rats were divided into four groups according to the all-trans retinoic acid content of their diets: control group (n=15), chow not enriched with all-trans retinoic acid; chow enriched with 0.3mg of all-trans retinoic acid per kilogram (n=13); chow enriched with 10mg of all-trans retinoic acid per kilogram (n=15); and chow enriched with 50mg of all-trans retinoic acid per kilogram (n-15). After 30 days of this diet, the animals were killed, their left paws were frozen and dissected and the tibias were submitted to the flexion study which assessed maximum force and shear modulus. One-way analysis of variance was used with significance set at p<0.05.
Results
The mean maximum force values in newtons (SD) were: control group =37.94, SD=4.76; 0.3mg group = 36.49, SD= 4.38; 10mg group = 40.12, SD=6.03; 50mg group =35.68, SD=5.22 (p=0.107). The mean shear modulus values (SD) in newtons/millimeter were: control group =31.84, SD=6.75; 0.3mg group =29.18, SD=4.35; 10mg group =35.48, SD=8.14; 50mg group =30.31, SD=7.14 (p=0.85).
Conclusion
Biomechanical studies showed that different doses of all-trans retinoic acid for 30 days had no effect on young rats’ bone resistance.

References

Dawson MI. The importance of vitamin A in nutrition. Curr Pharm Des. 2000; 6(3):311-25.

Fairfield KM, Fletcher RH. Vitamins for chronic disease prevention in adults: scientific review. JAMA. 2002; 287(23):3116-26.

Ramalho RA, Flores H, Saunders C. Hypovitaminosis A in Brazil: a public health problem. Rev Panam Salud Publica. 2002; 12(2):117-22.

Qiao YL, Dawsey SM, Kamangar F, Fan JH, Abnet CC, Sun XD, et al. Total and cancer mortality after supplementation with vitamins and minerals: follow-up of the Linxian General Population Nutrition Intervention Trial. J Natl Cancer Inst. 2009; 101(7):507-18.

Ganceviciene R, Zouboulis CC. Isotretinoin: state of the art treatment for acne vulgaris. J Dtsch Dermatol Ges. 2010; 8 (Suppl 1):S47-59.

Klamt F, Dal-Pizzol F, Roehrs R, Oliveira RB, Dalmolin R, Henriques JA, et al. Genotoxicity, recombinogenicity and cellular preneoplasic transformation induced by vitamin A supplementation. Mutat Res. 2003; 539(1-2):117-25.

Wolbach SB. Vitamin A deficiency and excess in relation to skeletal growth. J Bone Joint Surg. 1947; 29:171-92.

Binkley N Krueger D. Hypervitaminosis A and bone. Nutr Rev. 2000; 58:138-44.

Rhode CM, Deluca H. Bone resorption activity of all-trans retinoic acid is independent of vitamin D in rats. J Nutr. 2003; 133:777-83.

Morales-Torres J, Gutiérrez-Ureña S, Osteoporosis committee of Pan-American League of Associations for Rheumatology. The burden of osteoporosis in Latin America. Osteoporos Int. 2004; 15(8):625-32.

Lanzillotti HS, Lanzillotti RS, Trotte APR, Dias AS, Bornand B, Costa EAMM. Osteoporose em mulheres na pós-menopausa, cálcio dietético e outros fatores de risco. Rev Nutr. 2003; 16(2):181-93. doi: 10.1590/S1415-52732003000200005.

Melhus H, Michaelsson K, Kindmark A, Bergstrom R, Holmberg L, Mallmin H, et al. Excessive dietary intake of vitamin A is associated with reduced bone mineral density and increased risk for hip fracture. Ann Intern Med. 1998; 129:770-8.

Feskanich, D, Singh, V, Willett, WC, Colditz GA. Vitamin A intake and hip fractures among postmenopausal women. JAMA. 2002; 287:47-54.

Johanson S, Lind PM, Hakansson H, Oxlund H, Örberg J, Melhus H. Subclinical Hypervitaminosis A causes fragile bones in rats. Bone. 2002; 31(6): 685-9.

Reeves GP, Forrest HN, Fahey CG. AIN-93 purified diets for laboratory rodents: final report of the Americam Institute of Nutrition Ad Hoc writing committee on the reformulation of the AIN-76 A rodent diet. J Nutr. 1993; 123:1939-51.

Mattila PT, Svanberg, MJ, Jãmsã T, Knuuttila MLE. Improved Bone Biomechanical Properties in XylitolFed Aged Rats. Metabolism. 2002; 51(1):92-6.

StatSoft Inc. STATISTICA (data analysis software system) version 6.0. Tulsa: StatSoft; 2001.

Anderson JJB. Oversupplementation of vitamin A and osteoporotic fractures in the elderly: to supplement or not to supplement with vitamin A. J Bone Miner Res. 2002; 17:1359-62.

Denke MD. Dietary retinol: a double-edged sword. JAMA. 2002; 287:102-4.

Frame B, Jackson CE, Reynolds WA, Umphrey JE. Hypercalcemia and skeletal effects in chronic hypervitaminosis A. Ann Inter Med. 1974; 80:44-8.

Jowsey J, Riggs BL. Bone changes in a patient with hypervitaminosis A. J Clin Endocrinol Metab. 1968; 28:1833-5.

Ragavan VV, Smith JE, Bilezekian JP. Vitamin A toxicity and hipercalcemia. Am J Med Sci. 1982; 283:161-4

Frankel TL, Seshadri MS, McDowall DB, Cornish CJ. Hypervitaminosis A and calcium-regulating hormones in rat. J Nutr. 1986; 116:578-87.

Leelaprute V, Boonpucknavig V, Bhamarapravati N, Weerapradist W. Hypervitaminosis A in rats. Arch Pathol Lab Med. 1973; 96:5-9.

Moore T, Wang YL. Hypervitaminosis A. Biochem J.1945; 39:222-8.

Högström M, Nordström A, Nordström P. Retinol, retinol-binding protein 4, abdominal fat mass, peak bone mineral density, and markers of bone metabolism in men: the Northern Osteoporosis and Obesity (NO2) Study. Eur J Endocrinol. 2008; 158(5):765-70.

Kawahara TN, Krueger DC, Engelke JA, Harke JM, Birkley NC. Short-Term Vitamin A supplementation does not affect bone turn over in men. J Nutr. 2002; 132:1169-72.

Li XF, Dawson-Hughes B, Hopkins R, Russell RM, Jee WS, Bankson D, et al. The effects of chronic vitamin A excess on bone remodeling in aged rats. Proc Soc Exp Biol Med. 1989; 191:103-7.

Nieman C, Obbink H. The biochemistry and pathology od hipervitaminosis A. Vitam Horm. 1998; 12:69-99.

Hough S, Avioli LV, Muir H, Gelderblom D, Jenkins G, Kurasi H, et al. Effects of hypervitaminosis A on the bone and mineral metabolis of the rat. Endocrinology. 1988; 122:2933-9.

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Published

2023-08-31

How to Cite

SOUZA, L. B. de, FREIRE, C. M. M. ., ALMEIDA, R. N. A. de, MÜLLER, S. S. ., PAIVA, S. A. R. ., & MAZETO, G. M. F. da S. . (2023). Effect of varied doses of retinoic acid on young rats’ bone resistance. Brazilian Journal of Nutrition, 24(3). Retrieved from https://periodicos.puc-campinas.edu.br/nutricao/article/view/9432

Issue

Vol. 24 No. 3 (2011): Revista de Nutrição

Section

ORIGINAL ARTICLE

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Copyright (c) 2023 Luciana Bronzi de SOUZA, Cristiana Maria Murbach FREIRE, Renata Noêmia Alves de ALMEIDA, Sérgio Swain MÜLLER, Sérgio Alberto Rupp PAIVA, Gláucia Maria Ferreira da Silva MAZETO

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