High-fat diet using soybean meal as protein source: use for selecting rats prone and resistant to obesity
Keywords:
Fat deposit, Hyperlipidic diet, Soya flour, Obesity-prone and obesity-resistant, RatsAbstract
Objective
The objective was to develop a high-fat, low cost diet, using soybean meal as protein source. This diet should effectively discriminate between rats prone and resistant to obesity and allow the obese phenotype to be achieved in the animals that are prone to obesity. Furthermore, the diet must be palatable and not be rejected by the animal in the short run.
Methods
The chow was obtained by mixing the following ingredients: condensed milk (15.5%), peanuts (18.5%), soybean meal (20.0%), corn oil (6.0%), Bio Tec chow (30.0%) and chocolate wafer cookies (10.0%). In order to make it appropriate for rats, the mixture was pelleted and subjected to food analysis. The chow was offered to Wistar rats for a week. The animals were subsequently separated according to weight gained. The upper third group was considered prone to obesity and the lower third group was considered resistant to obesity. The animals were sacrificed 80 days later to determine body weight, food intake, retroperitoneal, periepididymal
and carcass fats and total fats.
Results
Food analysis found that the chow had an energy density of 5.31 Kcal/g, 22.3% fat, 22.2% protein, 15.9% fiber and 35.7% carbohydrates. After being fed for 87 days with the high-fat diet, obesity-prone rats had higher body weight and retroperitoneal, periepididymal and total fats than obesity-resistant animals (p<0.05). Food intake was also higher among obesity-prone rats (p<0.05). The replacement of casein by soybean meal as protein source reduced the cost of the study by 96.0%.
Conclusion
The substitution of casein by soybean meal in a high-fat diet allows cost reduction and the identification of obese-prone rats. Continuous use of this high-fat diet resulted in the development of the obese phenotype, as seen with other diets used in the literature.
References
Pereira LO, Francischi RP, Lancha AH. Obesidade: hábitos nutricionais, sedentarismo e resistência à insulina. Arq Bras Endocrinol Metab. 2003; 47 (2):111-27.
Korner J, Aronne LJ. The emerging science of body weight regulation and its impact on obesity treatment. J Clin Invest. 2003; 111(5):565-70.
Harris RBS. Factors influencing energy intake of rats fed either a high-fat or a fat mimetic diet. Int J Obes. 1994; 18(9):632-40.
Chang S, Graham B, Yakubu F, Lin D, Peters JC , Hill JO. Metabolic differences between obesityprone and obesity-resistant rats. Am J Physiol Reg Int Comp Physiol. 1990; 259(6):1103-10.
Woods SC, Seeley RJ, Rushing PA, Dálessio D, Tso P. A controlled high-fat diet induces an obese syndrome in rats. J Nutr. 2003; 133(5):1081-7.
Estadella D, Oyama LM, Dâmaso AR, Ribeiro EB, Oller CM. Effect of palatable hyperlipidic diet on lipid metabolism of sedentary and exercised rats. Nutrition. 2004; 20(2):218-24.
Duarte ACGO, Fonseca DF, Soave CF, Fiorese MS, Dâmaso AR. Dieta hiperlipídica e capacidade secretória de insulina em ratos. Rev Nutr. 2006; 19(3): 341-8. doi: 10.1590/S1415-52732006000300005.
Lucas F, Ackroff K, Sclafani A. Dietary fat-induced hyperphagia in rats as a function of fat type and physical form. Physiol-Behav. 1989; 45(5):937-46.
Ackroff K, Lucas F, Sclafani A. Flavor preference conditioning as a function of fat source. Physiol-Behav. 2005; 85(4):448-60.
Dourmashkin JT, Chang GQ, Hill JO, Gayles EC, Fried SK, Leibowitz SF. Model for predicting and phenotyping at normal weight the long-term propensity for obesity in Sprague-Dawley rats. Physiol Behav. 2006; 87(4):666-78.
Greenberg D, McCaffery J, Potack JZ, Bray GA, York DA. Differential satiating effects of fats in the smal intestine of obesity-resistant and obesity-prone rats. Physiol Behav.1999; 66(4):621-26.
Levin BE, Arcuate NPY. Neurons and energy homeostasis in diet-induced obese and resistant rats. Am J Physiol Regul Integr Comp Physiol. 1999; 276(2):R382-R7.
Levin BE, Hogan S, Sullivan AC. Initiation and perpetuation of obesity and obesity resistance in rats. Am J Physiol Regul Integr Comp Physiol. 1989; 256(3):R766-R71.
Levin BE, Keesey RE. Defense of differing body weight set points in diet-induced obese and resistant rats. Am J Physiol Regul Integr Comp Physiol. 1998; 274(3):R412-9.
York DA. Genetic models of animal obesity. In: Björntorp P, Brodoff BN, editors, Obesity. Philadelphia: J.B. Lippincott Company; 1992. p.233-40.
MacLean PS, Higgins JA, Johnson GC, FlemingElder BK, Peters JC, Hill JO. Metabolic adjustments with the development, treatment, and recurrence of obesity in obesity-prone rats. Am J Physiol Regul Integr Comp Physiol. 2004; 287(2):R288-97.
Marques-Lopes I, Marti A, Moreno-Aliaga A. Aspectos genéticos da obesidade. Rev Nutr. 2004; 17(3):327-38. doi: 10.1590/S1415-52732004000300006.
Willet W. Is dietary fat a major determinant of body fat? Am J Clin Nutr. 1998; 67:556-62.
Nascimento AF, Sugisaki MM, Leopoldo A, LimaLeopoldo AP, Luvizotto RAM, Nogueira CR. A Hypercaloric pellet-dietcycles induces obesity and co-morbidities in Wistar Rats. Arq Bras Endocrinol Metabol. 2008; 52(8):968-74. doi: 10.1590/S000427302008000600007.
Monteiro JBR, Costa NMB, Milagres EKH. Avaliação da qualidade proteica de dois formulados em pó, à base de soja enriquecidos com zinco, selênio e magnésio para utilização em nutrição enteral. Ciênc Tecnol Aliment. 2004; 24(1):6-10. doi: 10.1590/S0101-20612004000100002.
Silva MS, Naves MMV, Oliveira RBO, Leite SM. Composição química e valor proteico do resíduo de soja em relação ao grão de soja. Cienc Tecnol Aliment. 2006; 26(3):571-6. doi: 10.1590/S0101-20612006000300014.
Association of Official Analytical Chemists. Official Methods of Analysis. 15th ed. Arlington: AOAC; 1990.
Jansen GR, Zanetti ME, Hutchison CF. Studies in lipogenesis in vivo. Fatty acid and cholesterol synthesis during starvation and refeeding. Biochem J. 1966; 101(3):811-8.
Levin BE, Dunn-Meynell ADM. Defense of body weight against chronic caloric restriction in obesityprone and -resistant rats. Am J Physiol Regul Integr Comp Physiol. 2000; 278(1):R231-7.
Commerford SR, Pagliassotti MJ, Melby CL, Wei Y, Hill JO. Inherent capacity for lipogenesis or dietary fat retention is not increased in obesity-prone rats. Am J Physiol Regul Integr Comp Physiol. 2001; 280(6):1680-7.
Levin BE, Dunn-Meynell AA, Ricci MR, Cummings DE. Abnormalities of leptin and ghrelin regulation in obesity-prone juvenile rats. Am J Physiol End Metab. 2003; 285(5):949-57.
Takes NC, Levin BE. Obesity-prone rats have preexisting defects in their counterregulatory response to insulin-induced hipoglycemia. Am J Physiol Regul Integr Comp Physiol. 2004; 287(5): 1110-5.
Lavigne C, Marette A, Jacques H. Cod and soy proteins compared with casein improve glucose tolerance and insulin sensitivity in rats. Am J Physiol Endocrinol Metab. 2000; 278(3):E491-E500.
Himaya A, Fantino M, Antoine JM, Brondel L, Louis-Sylvestre J. Satiety power of dietary fat: a new appraisal. Am J Clin Nutr. 1997; 65(5):1410-8.
Levin BE, Dunn-Meynell ADM. Defense of body weight depends on dietary composition and palatability in rats with diet-induced obesity. Am J Physiol Regul Integr Comp Physiol. 2002; 282(1): R46-R54.
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Copyright (c) 2023 Ludimila Canuto CABEÇO, Mayumi AKIBA, Marcela Silva CALSA, Denise Rangel da Silva SARTORI, Maria de Lourdes Mendes VICENTINI-PAULINO, Daniela Felipe PINHEIRO
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