Prevention of oxidative stress in renal ischemia-reperfusion syndrome in rats with nutritional antioxidant supplementation
Keywords:
Antioxidants, Oxidative stress, Free radicals, Reperfusion injury, VitaminsAbstract
Objective
The objective of this study was to verify the potential protective effect of antioxidant vitamin supplementation in a model of renal ischemic-reperfusion injury in rats.
Methods
Twenty-nine Wistar rats were divided into three groups: groups I and II (n=10 each), were submitted to 60 minutes of renal ischemia, followed by 10 minutes of reperfusion; additionally, animals of group II were treated for twelve days with antioxidant vitamins (vitamin C 11.43mg/kg and vitamin E 28.5 mg/kg) before being submitted to ischemia; In group III (n=9), the animals were treated like the other groups but not submitted to ischemic-reperfusion injury and not given antioxidant supplements. Subsequently, blood samples and the kidneys were collected for assessment of malondialdehyde, uric acid and total antioxidant capacity.
Results
The malondialdehyde and uric acid of group I was significantly higher than those of group III (p<0.01), which in turn did not differ from group II. The levels of total antioxidant capacity of the animals of group I was lower than those of groups II and III (p<0.01).
Conclusion
These data confirmed the effective participation of oxidative stress in this model of renal ischemia-reperfusion syndrome in rats and showed that the use of antioxidant supplementation can protect the animals from oxidative changes.
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