Centesimal composition and protein nutritive value of yeast from ethanol fermentation and of yeast derivatives

Authors

  • Eunice Akemi YAMADA Instituto de Tecnologia de Alimentos
  • Izabela Dutra ALVIM Instituto de Tecnologia de Alimentos
  • Marjorie Carelli Costa SANTUCCI Instituto de Tecnologia de Alimentos
  • Valdemiro Carlos SGARBIERI Instituto de Tecnologia de Alimentos

Keywords:

Saccharomyces, integral cells, autolysate, extract, protein concentrate, Saccharomyces cerevisiae proteins, fermentation

Abstract

The objective of this work was to promote the autolysis and the fractionation of the yeast (Saccharomyces sp.) for the production of autolysate and extract, as well as phosphorylated protein concentrate, from ethanol distillery yeast. Comparative studies of centesimal composition, essential amino acid profiles and protein nutritive value were performed for the unprocessed integral cells, and for autolysate, extract and phosphorylated protein concentrate. Protein and carbohydrate (dietary fiber) were the main components for the integral cells and autolysate. For the extract and the protein concentrate the main components were protein and minerals (ashes). The autolysate and the integral cells presented the highest essential amino acid indexes, followed by the protein concentrate and the extract. Protein digestibility ranged from 68% (integral cells) to 91% (extract). Net protein ratio ranged from 2.1 (integral cells) to 4.3 (casein-reference). There was no statistical difference in Net protein ratio among autolysate, extract and protein concentrate (3.9 to 4.2). The protein concentrate promoted the highest growth in the period (21 days), followed by the extract and the autolysate. The integral cells showed the lowest capacity to promote rat growth.

References

Dziezak JD. Yeast and yeast derivatives: definitions, characteristics and processing. Food Technol 1987; 42(2):104-21.

Kinight S. Yeast protein enhances flavour and nutrition. Food Process 1986; 55(10):13-4.

Halasz A, Lásztity R. Use of yeast biomass in food production. Boca Raton: CRC Press; 1991. 312p.

Peixoto N. Processamento de produtos de biomassa de levedura para alimentação humana; potencial, mercado interno e externo. In: Anais do Workshop Produção de Biomassa de Levedura: em alimentação humana e animal; 1996; Campinas. Campinas: Ital; 1996. p.90-8.

Furco AM. Produção de biomassa de levedura em destilarias de álcool. In: Anais do Workshop Produção de Biomassa de Levedura em

Alimentação Animal e Humana; 1996: Campinas. Campinas: Ital; 1996. p.52-8.

Butolo JE. Uso de biomassa de levedura em alimentação animal: propriedades, custo relativo a outras formas de nutrientes. In: Anais do Workshop Produção de Biomassa de Levedura em Alimentação Animal e Humana; Agosto, 1996: Campinas. Campinas: Ital; 1996. p.70-89.

Sgarbieri VC, Alvim ID, Vilela ISD, Baldini VLS, Bragagnolo N. Produção piloto de derivados de levedura (Saccharomyces sp.) para uso como ingrediente na formulação de alimentos. Braz J Food Technol 1999; 2(1/2):119-25.

Caballero-Córdoba GM, Sgarbieri VC. Nutritional and toxicological evaluation of yeast (Saccharomyces cerevisiae) biomass and a yeast protein concentrate. J Sci Food Agric 2000; 80:341-51.

Vilela ESD, Sgarbieri VC, Alvim ID. Determinação do valor protéico de células íntegras, autolisado total e extrato de levedura (Saccharomyces sp.). Rev Nutr 2000; 13(3):185-92.

Vilela ESD, Sgarbieri VC, Alvim ID. Valor nutritivo da biomassa de células íntegras, do autolisado e do extrato de levedura originária de cervejaria. Rev Nutr 2000; 13(2):127-34.

Association of Official Agricultural Chemists. Official Methods of Analysis. 16th ed. Washington DC; 1998.

Bligh EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911-7.

Asp N, Johansson C.G., Halmer H, Siljeström MA. A rapid enzymatic assay of insoluble and soluble dietary fiber. J Agric Food Chem 1983; 31(3): 476-82.

Herbert D, Phipps PJ, Stranger RE. Chemical analysis of microbial cells. In: Noris JR, Ribbors PW, editors. Methods of enzymology. London: Academic Press; 1971. v.5B.

Spies JR. Determination of tryptophan in proteins. Anal Chem 1967; 39(10):1412-15.

Reeves PG, Nielsen FH, Fahey Jr CC. AIN-93 purified diet for laboratory rodents: final report of the American Institute of Nutrition ad hoc Committee on the reformulation of the AIN-76a rodent diet. J Nutr 1993; 123(2):467-72.

Food and Agriculture Organization. Protein quality evaluation. Rome; 1989. 72p. (Report of a joint FAO/WHO Expert Consultation, Food and Nutrition Paper, n.51).

Henley EC, Kuster JM. Protein quality evaluation by protein digestibility corrected amino acid scoring. Food Technol 1994; 48(4):74-7.

Pacheco MTB. Propriedades funcionais, nutricionais e toxicológicas de concentrados protéicos de levedura (Saccharomyces sp.) obtidos por diferentes processos de extração [tese]. Faculdade de Engenharia de Alimentos, UNICAMP; 1996. 158p.

Snider HE. Microbial sources of protein. Adv Food Res 1970; 18:85-91.

Galvez A, Ramírez MJ, Garcia-Garibay M. Chemical composition of a mixture of single cell protein obtained from Kluyveromyces fragilis and whey proteins. Arch Latinoam Nutr 1990; 40(2): 252-62.

Zinsly PF, Sgarbieri VC, Pereira Dias NFG, Jacobucci HB, Pacheco MTB, Baldini VLS. Produção piloto de concentrados de proteínas de leite bovino: composição e valor nutritivo. Braz J Food Technol 2001; 4:1-8.

Published

2003-12-25

How to Cite

Akemi YAMADA, E., Dutra ALVIM, I. ., Costa SANTUCCI, M. C. ., & SGARBIERI, V. C. (2003). Centesimal composition and protein nutritive value of yeast from ethanol fermentation and of yeast derivatives. Brazilian Journal of Nutrition, 16(4). Retrieved from https://periodicos.puc-campinas.edu.br/nutricao/article/view/9154

Issue

Section

ORIGINAL ARTICLE