Hypolipidemic effect of camu-camu juice in rats
Keywords:
Dyslipidemia, Quercetin, RatsAbstract
Objective
This study evaluated the potential hypolipidemic effect of camu-camu juice (Myrciaria dubia (Kunth) McVaugh) in dyslipidemic rats.
Methods
Seventy-two adult male Wistar rats (Rattus norvegicus var. albinus Berkenhout) with an average weight of 200g were used. The experiment was divided into two phases: induction of dyslipidemia and treatment. Dyslipidemia was induced by a high-fat diet (commercial feed plus 10.0% lard, 1.0% cholesterol and 0.1% cholic acid) given to the animals for 21 days. In the treatment phase, 40 dyslipidemic rats were randomly divided into five groups (n=8). Of these, three were subjected to different treatments with camu-camu juice: 0.4mL.kg-1, 4.0mL.kg-1 and 10mL.kg-1 for 14 days. The other two groups were kept as parameters: a basal group (normolipidemic) and a hyperlipidemic group receiving 10mL.kg-1 of quercetin (standard treatment). The potential hypolipidemic effect of camu-camu juice was assessed by the following measurements: plasma lipoproteins (triacylglycerol, total cholesterol, high density lipoprotein-cholesterol and low density lipoproteincholesterol), fecal and liver cholesterol, and measurements of feed intake and body weight.
Results
All dosages of camu-camu juice were hypolipidemic, reducing triacylglycerol, total cholesterol, fecal cholesterol excretion and hepatic cholesterol. The best results were obtained by the 10 mL.kg-1 dosage of camu-camu juice. Rat body weight and food intake did not vary significantly during the treatment.
Conclusion
Camu-camu juice has a hypolipidemic effect in dyslipidemic rats. These results can be used as reference for future studies on this Amazonian fruit.
References
Sociedade Brasileira de Cardiologia. IV Diretriz Brasileira sobre dislipidemias e prevenção da aterosclerose do Departamento de Aterosclerose da Sociedade Brasileira de Cardiologia. Arq Bras de Cardiol. 2007; 88(Supl.1):2-19.
Costa RP, Menendez G, Bricarello LP, Elias MC, Ito M. Óleo de peixe, fitoesteróis, soja e antioxidantes: impactos nos lipídios e aterosclerose. Rev Soc Cardiol de São Paulo. 2000; 10(2):819-32.
Mcnamara DJ. Dietary cholesterol and atherosclerosis. Biochim Biophys Acta Mol Cell Biol Lipids. 2000; 1529(13):310-20.
Rodrigues HG, Diniz YS, Faine LA, Fernandes AAH, Novelli ELB. Suplementação nutricional com antioxidantes naturais: efeito da rutina na concentração de HDL-col. Rev Nutr. 2003; 16(3):315-20. doi: 101590/S1415-52732003000300009.
Flores WBC. Fruteiras diversas: a importância econômica do camu-camu. Toda Fruta. 1998; 3(1): 36-7.
Yuyama K, Aguiar JPL, Yuyama LKO. Camu-camu: um fruto fantástico como fonte de vitamina C. Acta Amaz. 2002; 32(1):169-74.
Yuyama LKO, Aguiar JPL, Yuyama K, Lopes TM, Fávaro DIT, Bergl PCB, et al. Teores de elementos minerais em algumas populações de camu-camu. Acta Amaz. 2003; 33(4):459-554.
Maeda RN, Andrade JS. Aproveitamento do camucamu (Myrciaria dubia) para produção de bebida alcoólica fermentada. Acta Amaz. 2003; 33(3): 489-98.
Manach C, Scalbert A, Morand C, Rémésy C, Jime´nez L. “Polyphenols: food sources and bioavailability”. Am J Clin Nutr. 2004; 79(5):727-47.
Lopes RM, Oliveira TT, Nagem TJ, Pinto AS. Flavonóides: farmacologia de flavonóides no controle hiperlipidêmico em animais experimentais. Biotecnologia Cien Desenvolv. 2000; 3(17):18-22.
Lin BB, Chen HL, Huang PC. Effects on instant Pauchong Tea, Cathecin, and Caffeine os serum cholesterol and serum low-density-lipoprotein in mice. Nutr Rep Int. 1986; 34:821-9.
Hladovec J. The effect of antithrombotics in a new model of arterial thrombosis. Thromb Res. 1986; 41(5):665-70.
Kirk EA, Sutherland P, Wang SA, Shait A, Leboeuf RC. Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL-receptor-deficient Mice. J Nutr. 1998; 128(6): 954-9.
Lin M, Anderson H, Flavin MT, Pai YS, MataGreenwood E, Pengsupap T, et al. In vitro anti-HIV activity of bioflavonoids isolated from rhus succedanea and garcania miltiflora. J Nat Prod. 1997; 60(9): 884-8.
Association of Official Analytical Chemists. Official methods of analysis. 12th ed. Washington (DC): AOAC; 1995.
Instituto Adolfo Lutz. Normas analíticas, métodos químicos e físicos para análises de alimentos. São Paulo; 1975.
Lees DH, Francis FJ. Standartization of pigment analyses in cranberries. Hort Science.1972; 7(1): 83-4.
Ranganna S. Analysis and quality control for fruit and vegetable products. New Delhi (India):Tata McGraw-Hill; 1986.
Mazalli MR, Saldanha T, Bragagnolo N. Determinação de colesterol em ovos: comparação entre um método enzimático e um método por cromatografia líquida de alta eficiência. Rev Inst Adolfo Lutz. 2003; 62(1):49-54.
Teixeira AS, Chaves LS, Yuyama K. Esterases no exame da estrutura populacional de camu-camu (Myrciaria dubia (Kunth) McVaugh-Myrtaceae). Acta Amaz. 2004; 34(1):89-96.
Justi KC, Visentainer JV, Souza NE, Matsushita M. Nutricional composition and vitamin C stability in stored camu-camu (Myrciaria dubia) pulp. Arch Latinoam Nutr. 2000; 50(4):405-8.
Maeda RN, Pantoja L, Yuyama LKO, Chaar JM. Determinação da formulação do néctar de camucamu (Myrciaria dúbia McVaugh). Ciênc Tecnol Aliment. 2006; 26(1):70-4.
Alves RE, Filgueiras HAC, Moura CFH, Araújo NCC, Almeida AS. Camu-camu (Myrciaria dubia McVaugh): a rich natural source of vitamin C. Proc Interamer Soc Trop Hort. 2002; 46:11-3.
Nijveldt RJ, van Nood E, van Hoorn ECD, Boelens PG, Norren KK, Leeuwen PAML. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74(4): 418-25.
Pierpoint WS. Flavonoids in the human diet. Prog Clin Biol Res. 1986; 213: 125-40.
Kohrle J, Spank M, Cody V, Irmascher K, Prucher H, Hesch RD. Characteristics of a new synthetic thyrinine antagonist. Ann Endocrinol. 1986; 47:58-65.
Pinto AS, Oliveira TT, Nagem TJ, Gomes SM, Costa NMB, Oliveira MGA. Ação de flavonóides sobre os níveis de lipídios em ratos tratados com triton e seus efeitos antioxidantes. Rev Esc Farm Odontol Alfenas. 1999; 21:21-8.
Tall AR. Metabolic fate of chylomicon phospholipids and apoproteins in the rat. J Clin Invest. 1979; 64(4): 977-89.
Chajeck T, Eisenberg S. Very low density lipoprotein. Metabolism of phospholipids, cholesterol and apolipoprotein C in the isolated perfused rat heart. J Clin Invest. 1978; 61(6):1654-65
Scalbert A, Willianson G. Dietary intake and bioavailability of polyphenols. J Nutr. 2000; 130(8): 2073S-85S.
Aprikian O, Busserolles J, Manach C, Mazur A, Morand C, Davicco MJ, et al. Lyophilised apple counteracts the development of hypercholesterolemia, oxidative stress, and renal dysfunction in obese Zucker rats. J Nutr. 2002; 132(7):1969-76.
Li F, Li W, Fu W, Zhang Q, Koike K. Pancreatic LipaseInhibiting Triterpenoid Saponins from Fruits of Acanthopanax senticosus. Chem Pharm Bull. 2007; 55(7):1087-9
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Copyright (c) 2023 Maíra Cássia SCHWERTZ, Jeniffer Resende Patrocínio MAIA, Risonilce Fernandes Silva de SOUSA, Jaime Paiva Lopes AGUIAR, Lucia Kiyoko Ozaki YUYAMA
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