Acute caffeine intake lowers glycemia before and after acute physical exercise in diabetic rats
Keywords:
Caffeine, Diabetes Mellitus, Exercise, GlycemiaAbstract
Objective
The present study investigated the effects of caffeine supplementation combined with acute physical exercise on the glycemic response of diabetic rats.
Methods
Thirty-two 60-day-old rats with a mean weight of 238±3 g were divided into four groups: control, control caffeine, diabetes, and diabetes/caffeine. Diabetes was induced by 60 mg/kg of streptozotocin intraperitoneally. The control groups received an acute dose of caffeine (6 mg) or saline 60 minutes before exercise. The animals were then forced to swim for 60 minutes carrying a ballast weighing 6% of their body weight, producing lactacidemia compatible with the maximum lactate production during the steady state (5.5 mmol/L). After the acute exercise session, the animals were sacrificed and their blood collected for glucose analysis. The cardiovascular responses were measured before and after supplementation by tail cuff plethysmography. The one-way Analysisof Variance (Anova) was realized with post hoc of Student-Newman-Keuls to analyse the statistical differences between the supplementations, considering p<0.05.
Results
Caffeine at a dose of 6 mg/kg did not change the cardiovascular responses. However, compared with the control groups, caffeine reduced the blood glucose (42%, p<0.05) of diabetic rats after 60 minutes of exercise.
Conclusion
Acute caffeine ingestion together with exercise can increase glucose uptake without changing cardiovascular responses in animal models.
References
Brasil. Ministério da Saúde. Diabetes Mellitus. Brasília: Ministério da Saúde; 2006. Cadernos de Atenção Básica, n.16.
Nolan CJ, Damm P, Prentki M. Type 2 diabetes across generations: From pathophysiology to prevention and management. Lancet. 2011; 378(9786):169-81. doi: 10.1016/S0140-6736(11)60614-4
Graham TE, Battram DS, Dela F, El-Sohemy A, Thong FS. Does caffeine alter muscle carbohydrate and fat metabolism during exercise? Appl Physiol Nutr Metab. 2008; 33(6):1311-88. doi: 10.1139/H08-129
Vergauwen L, Richter EA, Hespel P. Adenosine exerts a glycogen-sparing action in contracting rat skeletal muscle. Am J Physiol. 1997; 272(5 Pt 1):762-8.
Graham TE. Caffeine and exercise, metabolism, endurance and performance. Sports Med. 2001; 31(11):785-807.
Paluska SA. Caffeine and exercise. Curr Sports Med Rep. 2003; 2(4):213-9.
Park S, Scheffler TL, Gunawan AM, Shi H, Zeng C, Hannon KM, et al. Chronic elevated calcium blocks AMPK-induced GLUT-4 expression in skeletal muscle. Am J Physiol Cell Physiol. 2009; 296(1):106- 15. doi: 10.1152/ajpcell.00114.2008
Conde SV, Silva TN, Gonzalez C, Carmo MM, Monteiro EC, Guarino MP. Chronic caffeine intake decreases circulating catecholamines and prevents diet-induced insulin resistance and hypertension in rats. Br J Nutr. 2012; 107(1):86-95. doi: 10.1017/S 0007114511002406
Yasuda N, Inoue T, Horizoe T, Nagata K, Minami H, Kawata T, et al. Functional characterization of the adenosine receptor contributing to glycogenolysis and gluconeogenesis in rat hepatocytes. Eur J Pharmacol. 2003; 459(2-3):159-66.
American Diabetes Association. Physical activity/ exercise and diabetes Mellitus. Diabetes Care. 2006; 29(1):1433-38. doi: 10.2337/diacare.27.2007.S58
American College of Sports Medicine. Exercise and type 2 diabetes. Med Sci Sport Exerc. 2010; 42(12): 2282-303. doi: 10.2337/dc10-9990
Burcelin R, Eddouks M, Maury J, Kande J, Assan R, Girard J. Excessive glucose production, rather than insulin resistance, accounts for hyperglycemia in recent-onset streptozocin-diabetic rats. Diabetologia. 1995; 35(3):283-90.
Gobatto CA, Mello MAR, Sibuya CY, Azevedo JRM, Santos LA, Kokubun E. Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem PhysiolMol Integr Phsiol. A. 2001; 130(1): 21-27.
Elayat AA, El-Naggar MM, Tahir M. An immunocytochemical and morphometric study of the rat pancreatic islets. J Anat. 1995; 186(Pt 3):629-37.
Park S, Jang JS, Hong SM. Long-term consumption of caffeine improves glucose homeostasis by enhancing insulinotropic action through islet insulin/insulin-like growth factor 1 signaling in diabetic rats. Metabolism. 2007; 56(5):599-607.
Chu YF, Chen Y, Black RM, Brown PH, Lyle BJ, Liu RH, et al. Type 2 diabetes-related bioactivities of coffee: Assessment of antioxidant activity, NF-κB inhibition, and stimulation of glucose uptake. Food Chem. 2011; 124(3):914-20. doi: 10.1016/j. foodchem.2010.07.019
Howarth FC, Al-Ali S, Al-Sheryani S, Al-Dhaheri H, Al-Junaibi SS, Almugaddum FA, et al. Effects of voluntary exercise on heart function in Streptozotocin (STZ): Induced diabetic rat. Int J Diabetes Metabol. 2007; 15(2):32-7.
Greenberg JA, Owen DR, Geliebter A. Decaffeinated coffee and glucose metabolism in young men. Diabetes Care. 2010; 33(2):278-80. doi: 10.2337/dc09-1539
Christ-Roberts CY, Pratipanawatr T, Pratipanawatr W. Exercise training increases glycogen synthase activity and GLUT4 expression but not insulin signaling in overweight nondiabetic and type 2 diabetic subjects. Metabolism. 2004; 53(9):1233-42.
Krebs JD, Parry-Strong A, Weatherall M, Carroll RW, Downie M. A cross-over study of the acute effects of espresso coffee on glucose tolerance and insulin sensitivity in people with type 2 diabetes Mellitus. Metabolism. 2012; 61(9):1231-7. doi: 10.1016/ j.metabol.2012.01.021
Noordzij M, Uiterwaal CSPM, Arends LR, Kok FJ, Grobbee DE, Geleijns JM. Blood pressure response to chronic intake of coffee and caffeine: A meta analysis of randomized controlled trials. J Hypertens. 2005; 23(5):921-8.
Nurminen ML, Niittynen L, Korpela R, Vapaatalo H. Coffee, caffeine and blood pressure: A critical review. Eur J Clin Nutr. 1999; 53(11):831-9.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Luiz Augusto da SILVA, Ricardo Aparecido PEREIRA, Janaína Angela TÚRMINA, Ivo Ilvan KERPPERS, Leandro Ricardo ALTIMARI, Carlos Ricardo Maneck MALFATTI
This work is licensed under a Creative Commons Attribution 4.0 International License.
