Effects of dietary high fructose corn syrup on regulation of energy intake and leptin gene expression in rats
Keywords:
Body weight, Diet, high-fat, Fatty acid synthase gene, Fructose, LeptinAbstract
Objective
To evaluate in Wistar rats the effect of chronic use of high fructose corn syrup on serum lipids, body weight, energy intake regulation, and expression of associated genes.
Methods
For 11 weeks, male rats were fed a standard diet with either water (control) or 15% high fructose corn syrup solution, or fed a high-fat diet. The rats’ food intake and body weight were measured weekly. Expression of leptin and fatty acid synthase genes was quantified in their brain and adipose tissue upon sacrifice at age 119 days using real-time polymerase chain reaction.
Results
The intake of 15% high fructose corn syrup did not affect the rats’ weight, only the rats on the high-fat diet gained significant weight. The rats in both diets had lower levels of leptin expression and high levels of fatty acid synthase in the brain, which were associated with high serum triglycerides.
Conclusion
Fifteen percent high fructose corn syrup intake and the high-fat diet reduced leptin gene expression in the brain of Wistar rats, with differential effects on weight gain.
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Copyright (c) 2023 Guadalupe LÓPEZ-RODRÍGUEZ, Silke Kotasek OSUNA, Marcos GALVÁN GARCÍA, Teodoro SUÁREZ DIEGUEZ
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