Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry

Autores

  • Thalles Pedrosa LISBOA Universidade Federal de Juiz de Fora
  • Leonã da Silva FLORES Universidade Federal de Juiz de Fora
  • Charlane Cimini CORREA Universidade Federal de Juiz de Fora
  • Rafael Arromba de SOUSA Universidade Federal de Juiz de Fora

Palavras-chave:

Atomic absorption, Chromium, Manganese, Micronutrients, Supplements, X-ray diffraction

Resumo

Objective
In this paper, we studied three different types of ordinary sports supplements containing whey protein: whey protein-based ones, hypercaloric ones, and protein bars.
Methods
A sample preparation procedure was studied employing microwave-assisted wet digestion in order to determine the Chromium and Manganese levels by graphite furnace atomic absorption spectrometry.
Results
The developed methods have presented good accuracy (recoveries in the range of 90% to 109%) and precision (Relative standard deviation <8%). Although an adequate detectability was obtained (50ng g-1 for Manganese and 65ng g-1 for Chromium), the sample preparation method was also adequate to inductively coupled plasma mass spectrometry analysis. The method was applied to 26 commercial samples, in which the Chromium concentrations were in the range between 0.22 and 1.0μg g-1 and the Manganese concentrations varied from 2.0 to 37μg g-1.
Conclusion
The results obtained by atomic absorption for both analytes were in agreement with those obtained by mass spectrometry. In addition, some samples presented concentrations of Chromium above the recommended daily intake and, as a result, we used the X-ray powder diffraction technique as an analytical tool to evaluate the oxidation state of Chromium in such samples.

Referências

Cerezo AB, Leal Á, Álvarez-Fernández MA, Hornedo-Ortega R, Troncoso AM, García-Parrilla MC. Quality control and determination of melatonin in food supplements. J Food Compost Anal. 2016;45:80-6.

Abdelraheem EMH, Hassan SM, Arief MMH, Mohammad SG. Validation of quantitative method for azoxystrobin residues in green beans and peas. Food Chem. 2015;182:246-50.

Krawczyk M. Determination of macro and trace elements in multivitamin dietary supplements by high-resolution continuum source graphite furnace atomic absorption spectrometry with slurry sampling. J Pharm Biomed Anal. 2014;88:377-84.

Hartmann C, Siegrist M. Benefit beliefs about protein supplements: a comparative study of users and non-users. Appetite. 2016;103:229-35.

Alves C, Lima RVB. Dietary supplement use by adolescents. J Pediatr. 2009;85(4):8.

Almeida MR, Souza LP, Cesar RS, Sousa RA, Izumi CMS. Investigation of sport supplements quality by Raman spectroscopy and principal component analysis. Vib Spectrosc. 2016;87:1-7.

Milani PG, Dacome AS, Nalesso CCF, Fiorenti CA, Costa CEM, Costa SC. Functional properties and sensory testing of whey protein concentrate sweetened with rebaudioside A. Rev Nutr. 2016;29(1):125-37. http://dx.doi.org/10.1590/1678-98652016000100012

Tokalıoğlu Ş, Clough R, Foulkes M, Worsfold P. Bioaccessibility of Cr, Cu, Fe, Mg, Mn, Mo, Se and Zn from nutritional supplements by the unified BARGE method. Food Chem. 2014;150:321-7.

Shinde UA, Sharma G, Xu YJ, Dhalla NS, Goyal RK. Insulin sensitising action of chromium picolinate in various experimental models of diabetes mellitus. J Trace Elem Med Biol. 2004;18(1):23-32.

Yao L, Zhu Y, Xu W, Wang H, Wang X, Zhang J, et al. Combination of dispersive solid phase extraction with dispersive liquid-liquid microextraction for the sequential speciation and preconcentration of Cr(III) and Cr(VI) in water samples prior to graphite furnace atomic absorption spectrometry determination. J Ind Eng Chem. 2019;72:189-95.

Sun H, Brocato J, Costa M. Oral chromium exposure and toxicity. Curr Environ Health Rep. 2015;2(3):295-303.

Andrade JK, Andrade CK, Felsner ML, Anjos VE. Ultrasound-assisted emulsification microextraction combined with graphite furnace atomic absorption spectrometry for the chromium speciation in water samples. Talanta. 2019;191:94-102.

Costello RB, Dwyer JT, Bailey RL. Chromium supplements for glycemic control in type 2 diabetes: limited evidence of effectiveness. Nutr Rev. 2016;74(7):455-68.

Nussbaumerova B, Rosolova H, Krizek M, Sefrna F, Racek J, Müller L, et al. Chromium supplementation reduces resting heart rate in patients with metabolic syndrome and impaired glucose tolerance. Biol Trace Elem Res. 2018;183(2):192-9.

Pham THN, Aitken JB, Levina A, Lay PA. Solid-state structural studies of chromium(III) nicotinate nutritional supplements. Inorg Chem. 2014;53(19):10685-94.

Bouabid S, Tinakoua A, Lakhdar-Ghazal N, Benazzouz A. Manganese neurotoxicity: behavioral disorders associated with dysfunctions in the basal ganglia and neurochemical transmission. J Neurochem. 2016;136(4):677-91.

Du S, Wu X, Han T, Duan W, Liu L, Qi J, et al. Dietary manganese and type 2 diabetes mellitus: two prospective cohort studies in China. Diabetologia. 2018;61(9):1985-95.

Horning KJ, Caito SW, Tipps KG, Bowman AB, Aschner M. Manganese is essential for neuronal health. Annu Rev Nutr. 2015;35(1):71-108.

Bjørklund G, Aaseth J, Skalny AV, Suliburska J, Skalnaya MG, Nikonorov AA, et al. Interactions of iron with manganese, zinc, chromium, and selenium as related to prophylaxis and treatment of iron deficiency. J Trace Elem Med Biol. 2017;41:41-53.

Marrero J, Rebagliati RJ, Leiva E, Londonio A, Smichowski P. Inductively coupled plasma optical emission spectrometric determination of fifteen elements in dietary supplements: are the concentrations declared in the labels accurate? Microchem J. 2013;108:81-6.

Pereira RM, Crizel MG, Novo DLR, Santos CMM, Mesko MF. Multitechnique determination of metals and non-metals in sports supplements after microwave-assisted digestion using diluted acid. Microchem J. 2019;145:235-41.

Garrido BC, Souza GHMF, Lourenço DC, Fasciotti M. Proteomics in quality control: whey protein-based supplements. J Proteom. 2016;147:48-55.

Agência Nacional de Vigilância Sanitária (Brasil). Resolução da Diretoria Colegiada n. 166: dispõe sobre a validação de métodos analíticos e dá outras providências. Brasília: Agência; 2017.

Almeida JS, Brandão GC, Santos GL, Teixeira LSG. Fast sequential determination of manganese and chromium in vegetable oil and biodiesel samples by high-resolution continuum source graphite furnace atomic absorption spectrometry. Anal Methods. 2016;8(15):3249-54.

Borges AR, François LL, Becker EM, Vale MGR, Welz B. Method development for the determination of chromium and thallium in fertilizer samples using graphite furnace atomic absorption spectrometry and direct solid sample analysis. Microchem J. 2015;119:169-75.

Zhong W-S, Ren T, Zhao L-J. Determination of Pb (Lead), Cd (Cadmium), Cr (Chromium), Cu (Copper), and Ni (Nickel) in Chinese tea with high-resolution continuum source graphite furnace atomic absorption spectrometry. J Food Drug Anal. 2016;24(1):46-55.

Dobrowolski R, Pawlowska-Kapusta I, Dobrzynska J. Chromium determination in food by slurry sampling graphite furnace atomic absorption spectrometry using classical and permanent modifiers. Food Chem. 2012;132(1):597-602.

Sola-Larrañaga C, Navarro-Blasco I. Chromium content in different kinds of Spanish infant formulae and estimation of dietary intake by infants fed on reconstituted powder formulae. Food Addit Contam. 2006;23(11):1157-68.

Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Brussels: Official Journal of the European Communities; 2002.

Pozzatti M, Borges AR, Dessuy MB, Vale MGR, Welz B. Determination of cadmium, chromium and copper in vegetables of the Solanaceae family using high-resolution continuum source graphite furnace atomic absorption spectrometry and direct solid sample analysis. Anal Methods. 2017;9(2):329-37.

Passos AS, Tonon GF, Nakadi FV, Mangrich AS, Andrade JB, Welz B, et al. Determination of Cr, Cu and Pb in industrial waste of oil shale using high-resolution continuum source graphite furnace atomic absorption spectrometry and direct solid sample analysis. Anal Methods. 2018;10(29):3645-53.

Welz B, Sperling M. Atomic absorption spectrometry. 3rd ed. Weinheim: Wiley-VCH; 1999.

Stilinović N, Škrbić B, Živančev J, Mrmoš N, Pavlović N, Vukmirović S. The level of elements and antioxidant activity of commercial dietary supplement formulations based on edible mushrooms. Food Funct. 2014;5(12):3170-8.

Avula B, Wang Y-H, Smillie TJ, Duzgoren-Aydin NS, Khan IA. Quantitative determination of multiple elements in botanicals and dietary supplements using ICP-MS. J Agri Food Chem. 2010;58(16):8887-94.

Krejčová A, Ludvíková I, Černohorský T, Pouzar M. Elemental analysis of nutritional preparations by inductively coupled plasma mass and optical emission spectrometry. Food Chem. 2012;132(1):588-96.

Silva AS, Brandao GC, Matos GD, Ferreira SLC. Direct determination of chromium in infant formulas employing high-resolution continuum source electrothermal atomic absorption spectrometry and solid sample analysis. Talanta. 2015;144:39-43.

Korfali SI, Hawi T, Mroueh M. Evaluation of heavy metals content in dietary supplements in Lebanon. Chem Cent J. 2013;7(1):10.

Brizio P, Benedetto A, Squadrone S, Tarasco R, Gavinelli S, Pellegrino M, et al. Heavy metals occurrence in Italian food supplements. Proceedings of the 16th International Conference on Heavy Metals in the Environment; 2012 Sep 23-27; Rome, Italy. Les Ulis: E3S Web of Conferences; 2013. http://dx.doi.org/10.1051/e3sconf/20130115006

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Publicado

18-08-2022

Como Citar

Pedrosa LISBOA, T. ., da Silva FLORES, L. ., Cimini CORREA, C. ., & Arromba de SOUSA, R. . (2022). Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry. Revista De Nutrição, 33, 1–13. Recuperado de https://periodicos.puc-campinas.edu.br/nutricao/article/view/6639

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ARTIGOS ORIGINAIS