Effects of perinatal protein restriction on the oxidative balance in the hypothalamus of 60-day-old rats
Keywords:
Hypothalamus, Oxidative stress, Protein-energy malnutritionAbstract
Objective
Evaluate the effects of maternal low-protein diet on the oxidative stress in the hypothalamus of 60-day-old rats.
Methods
Male Wistar rats were divided into two experimental groups according to the mother’s diet during pregnancy and lactation; control group (NP:17% casein n=6) and a malnourished group (LP:8% casein n=6). At 60 days of life, the rats were sacrificed for the collection of the hypothalamus for further biochemical analysis.
Results
Our results showed an increase in oxidative stress in malnourished group, observed through an increase in carbonyl content (p=0.0357), a reduction in the activity of the glutathione- S-transferase enzyme (p=0.0257), and a reduction in the non-enzymatic antioxidant capacity evidenced by the decrease in the ratio reduced glutathione/oxidized glutathione (p=0.0406) and total thiol levels (p=0.0166).
Conclusion
A low-protein diet during pregnancy and lactation is closely associated with increased oxidative stress and reduced antioxidant capacity in the hypothalamus of sixty-day-old rats.
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Copyright (c) 2023 Deyvison Guilherme Martins SILVA, Jonata Henrique de SANTANA, Thyago de Oliveira RODRIGUES, Elenilson Maximino BERNANDO, Anderson Apolonio da Silva PEDROZA, Cláudia Jacques LAGRANHA
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