Effect of subchronic oral administration of glucosamine in the regulation of body weight, glycemia and dyslipidemia induced hypercholesterolemic Wistar rat
Palavras-chave:
Blood glucose, Glucosamine, Cholesterol, HDL, Body weightResumo
Objective
This study evaluated the effect of oral glucosamine on overweight and dyslipidemia caused by a high-fat diet in rats.
Methods
our groups of Wistar rats: fed with commercial rodent food and drinking water without (control group) and with glucosamine (500 mg kg-1 per day) and a high-fat diet enriched with 24% (g/g) butter pork and drinking water without and with glucosamine, for 22 weeks; the body weight, abdominal fat, blood glucose, triglycerides, total cholesterol, and high density lipoprotein in serum were evaluated.
Results
Body weight gain, increased blood glucose levels and dyslipidemia were observed in the high-fat diet group versus the control group (p<0.001). When glucosamine was administered the same diet the effects were minimized, with a decrease in the amount of abdominal-fat and lipid profile levels in serum (p>0.05), regulated body weight, and high density lipoprotein and glycaemia (p<0.05). The glucosamine did not affect body weight and lipid metabolism in rats when administered with a normal diet.
Conclusions
Glucosamine regulated the body weight blood glucose and dyslipidemia caused by a high-fat diet, favoring increased high density lipoprotein cholesterol in rats. It did not affect body weight and lipid metabolism when administered with commercial food.
Referências
Siriwardhana N, Kalupahana NS, Cekanova M, Lemieux M, Greer B, et al. Modulation of adipose tissue inflammation by bioactive food compounds. J Nutr Biochem. 2013; 24(4):613-23. doi: 10.1016/j.jnutbio.2012.12.013
Carr MC, Brunzell JD. Abdominal obesity and dyslipidemia in the metabolic syndrome: Importance of type 2 diabetes and familialcombined hyperlipidemia in coronary artery disease risk. J Clin Endocrinol Metab. 2004; 89(6):2601-7. doi: 10.1210/jc.2004-0432
Brunzell JD, Ayyobi AF. Dyslipidemia in the metabolic syndrome and type 2 diabetes. Am J Med. 2003; 115(S8):S24-8. doi: 10.1016/j.amjmed.2003.08.011
Lemieux I, Pascot A, Couillard C, Lamarche B, Tchernof A, Almeras N, et al. Hypertriglyceridemic waist: A marker of the atherogenic metabolic triad (hyperinsulinemia; hyperapolipoprotein B; small, dense LDL) in men? Circulation. 2000; 102(2):179-84. doi: 10.1161/01.CIR.102.2.179
Aguilar SCA, Rojas R, Gómez PFJ, Valles V, Rios JM, Franco A, et al. High prevalence of metabolic syndrome in México. Arch Med Res. 2004; 35(1):76-81. doi: 10.1016/j.arcmed.2003.06.006
Kalupahana NS, Moustaid-Moussa N, Claycombe KJ. Immunity as a link between obesity and insulin resistance. Mol Aspects Med. 2012; 33(1):26-34. doi: 10.1016/j.mam.2011.10.011
Durrington P. Dyslipidaemia. Lancet. 2003; 362(9385):717-31. doi: 10.1016/S0140-6736(03) 14234-1
Wang S, Moustaid-Moussa N, Chen L, Mo H, Shastri A, Su R, et al. Novel insights of dietary polyphenols and obesity. J Nutr Biochem. 2014; 25(1):1-18. doi: 10.1016/j.jnutbio.2013.09.001
Velázquez MO, Rosas PM, Lara EA, Pastelín HG, Hernández C, Sánchez A, et al. Prevalencia e interrelación de enfermedades crónicas no transmisibles y factores de riesgo cardiovascular en México. Arch Cardiol Mex. 2003; 73(1):62-77.
Massiera F, Barbry P, Guesnet P, Joly A, Luquet S, Moreilhon-Brest C, et al. A Western-like fat diet is sufficient to induce a gradual enhancement in fat mass over generations. J Lipid Res. 2010; 51(8):2352-236. doi: 10.1194/jlr.M006866
Panchal SK, Brown L. Rodent models for metabolic syndrome research. J Biomed Biotechnol. 2011; 20011:351982. doi: 10.1155/2011/351982
Rolland V, Roseau S, Fromentin G, Nicolaidis S, Tomé D, Even PC. Body weight, body composition, and energy metabolism in lean and obese zucker rats fed soybean oil or butter. Am J Clin Nutr. 2002; 75(1):21-30.
Panchal SK, Poudyal H, Iyer A, Nazer R, Alam A, Diwan V, et al. High-carbohydrate, high-fat dietinduced metabolic syndrome and cardiovascular remodeling in rats. J Cardiovasc Pharmacol. 2011; 57(5):611-24. doi: 10.1097/FJC.0b013e3181feb90a
Lomba A, Milagro FI, Garcia-Diaz DF, Marti A, Campion J, Martinez JA. Obesity induced by a pairfed high fat sucrose diet: Methylation and expression pattern of genes related to energy homeostasis. Lipids Health Dis. 2010; 9:60. doi: 10.1186/1476-511X-9-60
Sever P, Dahlof B, Poulter N, Wedel H, Beevers G, Caulfield M, et al. Prevention of coronary and stroke events with atorvastatin in hypertensive patients who have average or lower-than-average cholesterol concentrations, in the Anglo-Scandinavian Cardiac Outcomes Trial-Lipid Lowering Arm (ASCOT-LLA): A multicenter randomized trial. Lancet. 2003; 361(9364):1149-58. doi: 10.1016/S0140-673 6(03)12948-0
Kwiterovich PO. The metabolic pathways of highdensity lipoprotein, low-density lipoprotein, and triglycerides: A current review. Am J Cardiol. 2000; 86(12A):L5-10. doi: 10.1016/S0002-9149(00)01461-2
McAlindon TE, LaValley MP, Gulin JP, Felson DT. Glucosamine and chondroitin for treatment of osteoarthritis: A systematic quality assessment and meta-analysis. Jama. 2000; 283(11):1469-75. doi:10.1001/jama.283.11.1469
Bondiolotti G, Bareggi SR, Frega NG, Strabioli S, Cornelli U. Activity of two different polyglucosamines, L112 and FF45, on body weight in male rats. Eur J Pharmacol. 2007; 567(1-2):155-8.
Bondiolotti G, Cornelli U, Strabbioli RS, Frega NG, Cornelli M, Bareggi S. Effect of a polyglucosamine on the body weight of male rats: Mechanisms of action. Food Chem. 2011; 124(3):978-82. doi: 10.1016/j.foodchem.2010.07.039
Magrans CT, Wilborn C, Rasmussen C, Ferreira M, Greenwood L, Campbell B, et al. Effects of diet type and supplementation of glucosamine, chondroitin, and MSM on body composition, functional status, and markers of health in women with knee osteoarthritis initiating a resistance-based exercise and weight loss program. J Int Soc Sports Nutr. 2011; 8(1):8. doi: 10.1186/1550-2783-8-8
Lamiaa A, Barakat A. Hypolipidemic and antiatherogenic effects of dietary chitosan and wheat bran in high fat-high cholesterol fed rats. Aust J Basic Appl Sci. 2011; 5(10):30-7.
Muurling M, Mensink RP, Pijl H, Romijn JA, Havekes LM, Voshol PJ. A fish oil diet does not reverse insulin resistance despite decreased adipose tissue TNFalpha protein concentration in ApoE-3*Leiden mice. J Nutr. 2003; 133(11):3350-5.
Anderson JW, Nicolosi RJ, Borzelleca JF. Glucosamine effects in humans: A review of effects on glucose metabolism, side effects, safety considerations and efficacy. Food Chem Toxicol. 2005; 43(2):187-201. doi: 10.1016/j.fct. 2004.11.006
Keita H, Ramírez-San Juan E, Paniagua-Castro N, Garduño-Siciliano L, Quevedo L. The long-term ingestion of a diet high in extra virgin olive oil produces obesity and insulin resistance but protects endothelial function in rats: A preliminary study. Hady Diabetol Metab Synd. 2013; 5:53 doi: 10.1 186/1758-5996-5-53
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinl Chem. 1972; 18(6):499-502.
Sánchez-Lasheras C, Könner AC, Brüning JC. Integrative neurobiology of energy homeostasisneurocircuits, signals and mediators. Front Neuroendocrinol. 2010; 31(1):4-15. doi: 10.1016/j. yfrne.2009.08.002
Laye MJ, Thyfault JP, Stump CS, Booth FW. Inactivity induces increases in abdominal fat. J Appl Physiol. 2007; 102(4):1341-7.
Fernández-Quintela A, Churruca I, Portillo MP. The role of dietary fat in adipose tissue metabolism. Public Health Nutr. 2007; 10(10A):1126-31.
Könner AC, Klöckener T, Brüning JC. Control of energy homeostasis by insulin and leptin: Targeting the arcuate nucleus and beyond. Physiol Behav. 2009; 97(5):632-8. doi: 10.1016/j.physbeh.2009.03.027
Marty N, Dallaporta M, Thorens B. Brain glucose sensing, counterregulation, and energy homeostasis. Physiology. 2007; 22:241-51.
Stein CJ, Colditz GA. The epidemic of obesity. J Clin Endocrinol Metab. 2004; 89(6):2522-5.
Hensrud DD. Diet and obesity. Curr Opin Gastroenterol. 2004; 20(2):119-24.
Stannard SR, Johnson NA. Insulin resistance and elevated triglyceride in muscle: More important for survival than thrifty genes? J Physiol. 2004. 554(Pt3):595-607. doi: 10.1113/jphysiol.2003.053926
Yu YH, Ginsberg HN. Adipocyte signaling and lipid homeostasis: Sequelae of insulin-resistant adipose tissue. Circ Res. 2005; 96(10):1042-52. doi: 10.1161/01.RES.0000165803.47776.38
Boden G. Obesity, insulin resistance and free fatty acids. Curr Opin Endocrinol Diabetes Obes. 2011; 18(2):139-43. doi: 10.1097/MED.0b013e3283444b09
Kahn SE, Hull RL, Utzschneider KM. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature. 2006; 444(7121):840-6.
Arner P. Human fat cell lipolysis: Biochemistry, regulation and clinical role. Best Pract Res Clin Endocrinol Metab. 2005; 19(4):471-82. doi: 10.1016/j.beem.2005.07.004
Frayn KN. Obesity and metabolic disease: Is adipose tissue the culprit? Proc Nutr Soc. 2005; 64(1):7-13. doi: org/10.1079/PNS2004403
Lemieux I, Poirier P, Bergeron J, Alméras N, Lamarche B, Cantin B, et al. Hypertriglyceridemic waist: A useful screening phenotype in preventive cardiology? Can J Cardiol. 2007; 23(Suppl. B):23B-31B.
Fujimoto VY, Kane JP, Ishida BY, Bloom MS, Browne RW. High-density lipoprotein metabolism and the human embryo. Hum Reprod. 2010; 16(1):20-38. doi: 10.1093/humupd/dmp029
Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). Jama. 2002; 285(2):2486-97. doi: 10.1001/jama.285.19.2486
Kong CS, Kim JA, Kim SK. Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes. Food Chem Toxicol. 2009; 47(10):2401-06. doi: 10.1016/j.fct.2009.06.010
Lechleitner M. Mitochondrial function role in insulin resistance and lipid metabolism. Acta Med Austriaca. 2004; 31(4):115-9.
Luo B, Parker GJ, Cooksey RC, Soesanto Y, Evans M, Jones D, et al. Chronic hexosamine flux stimulates fatty acid oxidation by activating AMP-activated protein kinase in adipocytes. J Biol Chem. 2007; 282(10):7172-80. doi: 10.1074/jbc.M607362200
Unger RH. Minireview: Weapons of lean body mass destruction: The role of ectopic lipids in the metabolic syndrome. Endocrinology. 2003; 144(12):5159-65. doi: 10.1210/en.2003-0870.5159
Lewis GF, Rader DJ. New insights into the regulation of HDL metabolism and reverse cholesterol transport. Circ Res. 2005; 96(12):1221-32.
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Copyright (c) 2023 Cornelio BARRIENTOS ALVARADO, Jorge SÁNCHEZ VÁZQUEZ, María Atanasia Silvia CÁRDENAS OSCOY, Osvaldo GARRIDO ACOSTA, Liliana ANGUIANO ROBLEDO
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
