Trombospondina na hipoplasia induzida por desnutrição protéica
Palavras-chave:
medula óssea, matriz extracelular, hipoplasia, desnutrição protéica, trombospondinaResumo
Objective
The objective of the present study was to measure the concentration of bone marrow extracellular matrix thrombospondin in mice, following hypoplasia induced by protein malnutrition.
Methods
Two-month-old male Swiss mice were submitted to protein malnutrition by way of a low-protein diet containing 4.0% casein until they lost 20.0% of their original body weight, while the control group mice were fed 14.0% casein for 15 days. The bone marrows of the animals were aspirated and transferred to phosphate-buffered saline tubes for extraction. The extracellular matrix protein was analyzed by 7.5% SDS-PAGE and thrombospondin by Enhanced Chemiluminescence Light Western blotting.
Results
The amount of thrombospondin was 30% higher in the undernourished samples when compared to the control samples.
Conclusion
This study suggests that the hypoplasia induced by protein malnutrition probably alters the functioning of the bone marrow microenvironment resulting in a higher thrombospondin concentration.
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Copyright (c) 2023 Cidônia de Lourdes VITURI, Márcio ALVAREZ-SILVA, Andréa Gonçalves TRENTIN, Vera Lúcia Cardoso Garcia TRAMONTE, Primavera BORELLI
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
