Efeito da goma guar parcialmente hidrolisada no metabolismo de lipídeos e na aterogênese de camundongos
Resumo
Objetivo
Observar os efeitos da goma guar parcialmente hidrolisada no metabolismo de colesterol e na formação de placa aterosclerótica em aorta de camundongos deficientes no receptor LDL, euglicêmicos ou com hiperglicemia induzida por estreptozotocina.
Métodos
Trinta e seis camundongos deficientes para o receptor de LDL foram divididos em quatro grupos de nove animais: grupos euglicêmicos, alimentados com dieta aterogênica padrão (controle euglicêmico) ou suplementada com 7,5% de goma guar parcialmente hidrolisada (goma guar parcialmente hidrolisada euglicêmico) e grupos hiperglicêmicos alimentados com dieta aterogênica padrão (controle hiperglicêmico) ou suplementada com 7,5% de goma guar parcialmente hidrolisada (goma guar parcialmente hidrolisada hiperglicêmico). Após quatro semanas de experimento foram medidos: ingestão alimentar, ganho de peso, glicemia, colesterol plasmático e hepático, assim como lesão aterosclerótica na aorta torácica e abdominal.
Resultados
Os resultados mostram que a suplementação de goma guar parcialmente hidrolisada levou ao aumento do colesterol hepático e plasmático em animais euglicêmicos, mas sem aumento na área de lesão aterosclerótica na aorta. Em animais hiperglicêmicos, a redução no colesterol plasmático não foi estatisticamente significante, mas no que se refere à lesão da aorta, observou-se redução significante.
Conclusão
Os resultados sugerem que a goma guar parcialmente hidrolisada pode reduzir a aterosclerose associada ao Diabetes Mellitus tipo 1.
Referências
National Cholesterol Education Program. Third report of the expert panel on detection, evaluation, and treatament of high blood cholesterol in adults. JAMA. 2001; 285:2496-9.
Juhan-Vague I, Alessi MC, Vague P. Increased plasma plasminogen activator inhibitor 1 levels. A possible link between insulin resistance and atherothrombosis. Diabetologia. 1991; 34(7): 457-62.
Izkhakov E, Meltzer E, Rubinstein A. Pathogenesis and management of diabetic dyslipidemia. Treat Endocrinol. 2003; 2(4):231-45.
Large V, Peroni O, Letexier D, Ray H, Beylot M. Metabolism of lipids in human white adipocyte. Diabetes Metab. 2004; 30(4):294-309.
Ishibashi S, Goldstein JL, Brown MS, Herz J, Burns DK. Massive xanthomatosis and atherosclerosis in cholesterol-fed low density lipoprotein receptor-negative mice. J Clin Invest. 1994; 93(5): 1885-93.
Lusis AJ. Atherosclerosis. Nature. 2000; 407(6801): 233-41.
Cascieri MA. The potential for novel anti-inflammatory therapies for coronary artery disease. Nat Rev Drug Discov. 2002; 1(2):122-30.
Press NA. Dietary reference intakes: proposed definition of dietary fiber. Washington: National Academy Press; 2001.
Slavin JL, Greenberg NA. Partially hydrolyzed guar gum: clinical nutrition uses. Nutrition. 2003; 19(6): 549-52.
Stanogias G, Pearce GR. The digestion of fibre by pigs. 3. Effects of the amount and type of fibre on physical characteristics of segments of the gastrointestinal tract. Br J Nutr. 1985; 53(3): 537-48.
Kishida T, Nogami H, Ogawa H, Ebihara K. The hypocholesterolemic effect of high amylose cornstarch in rats is mediated by an enlarged bile acid pool and increased fecal bile acid excretion, not by cecal fermented products. J Nutr. 2002; 132(9):2519-24.
Farmer JA, Gotto AM, Jr. Currently available hypolipidaemic drugs and future therapeutic developments. Baillieres Clin Endocrinol Metab. 1995; 9(4):825-47.
Alam NH, Meier R, Schneider H, Sarker SA, Bardhan PK, Mahalanabis D, et al. Partially hydrolyzed guar gum-supplemented oral rehydration solution in the treatment of acute diarrhea in children. J Pediatr Gastroenterol Nutr. 2000; 31(5):503-7.
Spapen H, Diltoer M, van Malderen C, Opdenacker G, Suys E, Huyghens L. Soluble fiber reduces the incidence of diarrhea in septic patients receiving total enteral nutrition: a prospective, double-blind, randomized, and controlled trial. Clin Nutr. 2001; 20(4):301-5.
Folch J, Lees GH, Sloane-Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957; 226(1):497-509.
Cheng HH, Lai MH. Fermentation of resistant rice starch produces propionate reducing serum and hepatic cholesterol in rats. J Nutr. 2000; 130(8):1991-5.
Alvarez-Leite J, Andrieux C, Ferrezou J, Riottot M, Vieira E. Evidences for absence of participation of the microbial flora in the hypocholesterolemic effect of guar gum in gnotobiotic rats. Comp Biochem Physiol. 1994; 109(2):503-10.
Alvarez-Leite JI, Andrieux C, Ferezou J, Riottot M, Vieira EC. Heat or gamma irradiation sterilization affects the hypocholesterolemic effect of guar gum in axenic and heteroxenic rats. Braz J Med Biol Res. 1994; 27(3):677-89.
Kondo S, Xiao J, Takahashi N, Miyaji K, Iwatsuki K, Kokubo S. Suppressive effects of dietary fiber in yogurt on the postprandial serum lipid levels in healthy adult male volunteers. Biosci Biotechnol Biochem. 2004; 68(5):1135-8.
Minekus M, Jelier M, Xiao J, Kondo S, Iwatsuki K, Kokubo S, et al. Effect of partially hydrolyzed guar gum (PHGG) on the bioaccessibility of fat and cholesterol. Biosci Biotechnol Biochem. 2005; 69(5):932-8.
Yamada K, Tokunaga Y, Ikeda A, Ohkura K, Mamiya S, Kaku S, et al. Dietary effect of guar gum and its partially hydrolyzed product on the lipid metabolism and immune function of SpragueDawley rats. Biosci Biotechnol Biochem. 1999;
(12):2163-7.
Yamada K, Tokunaga Y, Ikeda A, Ohkura K, Kaku-Ohkura S, Mamiya S, et al. Effect of dietary fiber on the lipid metabolism and immune function of aged Sprague-Dawley rats. Biosci Biotechnol Biochem. 2003; 67(2):429-33.
Favier M, Bost P, Guittard C, Demigne C, Remesy C. The cholesterol-lowering effect of guar gum is not the result of a simple diversion of bile acids toward fecal excretion. Lipids. 1997; 32(9): 953-9.
Weaver G, Tangel C, Krause J, Alpern H, Jenkins P, Parfitt M, et al. Dietary guar gum alters colonic microbial fermentation in azoxymethane-treated rats. J Nutr. 1996; 126(8):1979-91.
Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes. 2005; 54(6):1615-25.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001; 50(6):537-46.
Hansson GK, Libby P, Schonbeck U, Yan ZQ. Innate and adaptive immunity in the pathogenesis of atherosclerosis. Circ Res. 2002; 91(4):281-91.
Reeves PG, Nielsen FH, Fahey GCJ. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51.
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Copyright (c) 2023 Luciana Rodrigues FERNANDES, Marilene Diniz XISTO, Michele Groenner PENNA, Izabela Mascarenhas MATOSINHOS, Matheus Calab LEAL, Luciane Rodrigues PORTUGAL, Jacqueline Isaura Alvarez LEITE
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
