Qualitative and quantitative assessment of genetically modified soy in enteral nutrition formulas by polymerase chain reaction based methods
Palavras-chave:
Soy protein isolate, Enteral nutrition, Organisms genetically modified, Food traceability, Polymerase chain reactionResumo
Objective
The aim of this work was to investigate the occurrence of Roundup Ready soybean in enteral nutrition formulas sold in Brazil.
Methods
A duplex Polymerase Chain Reaction based on the amplification of the lectin gene and the construction of the recombinant deoxyribonucleic acid of transgenic glyphosate-tolerant soybean (35S promoter and chloroplast transit peptide gene) was performed in order to analyze the deoxyribonucleic acid obtained from nine soy protein isolate-containing formulas.
Results
Despite the highly processed nature of the food matrices, amplifiable deoxyribonucleic acid templates were obtained from all tested samples, as judged by the amplification of the lectin gene sequence. However, amplicons relative to the presence of Roundup Ready soybean were restricted to one of the nine enteral nutrition formulas analyzed as well as to the soybean reference powder, as expected. Quantitative analysis of the genetically modified formula by real-time Polymerase Chain Reaction showed a content of approximately 0.3% (w/w) of recombinant deoxyribonucleic acid from the Roundup Ready soybean.
Conclusion
The results show that one of the formulas contained genetically modified soy, pointing to the need of regulating the use of transgenic substances and of specific labeling in this product category.
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Copyright (c) 2023 Natália Eudes Fagundes de BARROS, Edna Maria Morais OLIVEIRA, Otniel Freitas SILVA, Joab Trajano SILVA, Vânia Margaret Flosi PASCHOALIN
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
