Efeito da redução de ninhada sobre as respostas autonômicas e metabólicas de ratos Wistar
Palavras-chave:
Glicemia, Insulina, Obesidade, Sistema nervoso autonômicoResumo
Objetivo
Este estudo investigou o perfil lipídico e a atividade elétrica dos nervos parassimpático (vago superior) e simpático (localizado na região esplâncnica) de ratos obesos oriundos de ninhada reduzida.
Métodos
Foram pesquisados dois grupos distintos, com 12 animas cada um: ninhada padrão, padronizado em nove filhotes por ninhada, e ninhada reduzida, três filhotes por ninhada. O consumo de ração e peso corporal foi acompanhado do desmame até o final do protocolo experimental. Aos 90 dias de idade, os animais foram anestesiados com (Thiopental®) e submetidos ao registro da atividade elétrica dos nervos simpático (vago) e parassimpático (da região esplâncnica); em seguida, foram sacrificados e retiradas e pesadas as gorduras retroperitoneal e periepididimal. Amostras de sangue foram coletadas para dosagens de glicemia, insulinemia, colesterol total, triglicerídeos e lipoproteína de alta densidade colesterol.
Resultados
Os ratos de ninhada reduzida apresentaram aumento da ingestão alimentar, peso corporal e tecido adiposo branco, quadros de hiperglicemia, hiperinsulinemia e hipercolesterolemia, aumento dos triglicérides e redução do lipoproteína de alta densidade colesterol.
Conclusão
Quanto à atividade do nervo vago, os ratos ninhada reduzida apresentaram um aumento significativo em relação aos ratos ninhada padrão, e mesmo não havendo diferença na atividade simpática, o modelo ninhada reduzida mostrou-se eficaz para indução da obesidade, dislipidemia, hipercolesterolemia, hiperinsulinemia, hiperglicemia e desequilíbrio autonômico em roedores.
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Copyright (c) 2023 Wilson RINALDI, Tatiane Aparecida da Silva RIBEIRO, Angélica Sbrolini MARQUES, Gabriel Sérgio FABRICIO, Laize Peron TÓFOLO, Rodrigo Mello GOMES, Fagner Cordeiro Vilar MENDES, Paulo Cezar de Freitas MATHIAS
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
