The effects of kefir in mixed meals on appetite and food intake: a randomized cross-over trial


  • Zeynep Caferoglu Erciyes University
  • Gizem Aytekin Sahin Erciyes University


Appetite, Glycemic index, Kefir, Obesity, Probiotics


The natural probiotic kefir is fermented milk, and may have effects on satiety and voluntary energy intake. This randomized crossover trial aimed to determine whether kefir, consumed with low- or high-glycemic index meals, affects appetite and subsequent food intake.
Twenty four healthy females aged 21-24 years, were recruited from Erciyes University and the surrounding community.
The participants were submitted to three different breakfasts: a low glycemic index accompanied by milk, a low glycemic index with kefir, and a high glycemic index with kefir, with a 1-week washout period between meals. At 0, 15, 30, 60, 90, 120, 150, and 180 minutes after the meal, appetite ratings were measured by the visual analog scale, and then ad libitum lunch was served.
No differences in appetite scores and voluntary energy intake were detected between the test meals. Furthermore, palatability ratings were similar between test meals, except for the higher score of high glycemic index kefir for overall palatability.
This study demonstrated that adding kefir to a high glycemic index meal may prevent increases in appetite and food intake, resulting in postprandial responses similar to those of a low glycemic index meal. These findings might enable the development of novel dietary strategies based on appetite regulation to treat or prevent obesity, particularly for Western societies. This trial was registered at under the process NCT03636217.


Augustin LS, Kendall CW, Jenkins DJ, Willett WC, Astrup A, Barclay AW, et al. Glycemic index, glycemic load and glycemic response: an International Scientific Consensus Summit from the International Carbohydrate Quality Consortium (ICQC). Nutr Metab Cardiovas. 2015;25:795-815.

Visuthranukul C, Sirimongkol P, Prachansuwan A, Pruksananonda C, Chomtho S. Low-glycemic index diet may improve insulin sensitivity in obese children. Pediatr Res. 2015;78:567-73.

Juanola-Falgarona M, Salas-Salvado J, Ibarrola-Jurado N, Rabassa-Soler A, Diaz-Lopez A, Guasch-Ferre M, et al. Effect of the glycemic index of the diet on weight loss, modulation of satiety, inflammation, and other metabolic risk factors: a randomized controlled trial. Am J Clin Nutr. 2014;100:27-35.

Wang ML, Gellar L, Nathanson BH, Pbert L, Ma Y, Ockene I, et al. Decrease in glycemic index associated with improved glycemic control among latinos with type 2 diabetes. J Acad Nutr Diet. 2015;115:898-906.

Klees M, Dong Y, Cao M. Reversal effects of probiotic supplementation on a high glucose diet in caenorhabditis elegans model. Graduate Research and Discovery Symposium. 2015:194.

Firouzi S. Probiotics and glycemic control: a simplified interplay model for the pathways behind. Food Health. 2018;1:30-6.

Pimenta FS, Luaces-Regueira M, Ton AM, Campagnaro BP, Campos-Toimil M, Pereira TM, et al. Mechanisms of action of kefir in chronic cardiovascular and metabolic diseases. Cell Physiol Biochem. 2018;48:1901-14.

Cerdó T, García-Santos JA, Bermúdez MG, Campoy C. The role of probiotics and prebiotics in the prevention and treatment of obesity. Nutrients. 2019;11:635-66.

Mazloom K, Siddiqi I, Covasa M. Probiotics: how effective are they in the fight against obesity? Nutrients. 2019;11:258-82.

Zoumpopoulou G, Pot B, Tsakalidou E, Papadimitriou K. Dairy probiotics: beyond the role of promoting gut and immune health. Int Dairy J. 2017;67:46-60.

Chowdhury EA, Richardson JD, Tsintzas K, Thompson D, Betts JA. Carbohydrate-rich breakfast attenuates glycaemic, insulinaemic and ghrelin response to ad libitum lunch relative to morning fasting in lean adults. Br J Nutr. 2015;114:98-107.

Vega-López S, Venn B, Slavin J. Relevance of the glycemic index and glycemic load for body weight, diabetes, and cardiovascular disease. Nutrients. 2018;10:1361-88.

Gibbons C, Blundell JE. Quantifying appetite and satiety. In: Krentz AJ, Weyer C, Hompesch M. Translational research methods in diabetes, obesity, and nonalcoholic fatty liver disease. Springer: Cham; 2019.

Leidy HJ, Todd CB, Zino AZ, Immel JE, Mukherjea R, Shafer RS, et al. Consuming high-protein soy snacks affects appetite control, satiety, and diet quality in young people and influences select aspects of mood and cognition. J Nutr. 2015;145:1614-22.

Stevenson EJ, Astbury NM, Simpson EJ, Taylor MA, Macdonald IA. Fat oxidation during exercise and satiety during recovery are increased following a low-glycemic index breakfast in sedentary women. J Nutr. 2009;139:890-7.

Akin L, Kendirci M, Narin F, Kurtoglu S, Saraymen R, Kondolot M, et al. The endocrine disruptor bisphenol a may play a role in the aetiopathogenesis of polycystic ovary syndrome in adolescent girls. Acta Paediatr. 2015;104:e171-7.

Shishehgar F, Mirmiran P, Rahmati M, Tohidi M, Tehrani FR. Does a restricted energy low glycemic index diet have a different effect on overweight women with or without polycystic ovary syndrome? Bmc Endocr Disord. 2019;19:93-104.

Zafar TA, Kabir Y, Ghazaii C. Low glycemic index foods suppress glycemic responses, appetite and food intake in young Kuwaiti females. Kuwait J Sci Eng. 2011;38:111-23.

Lennerz BS, Alsop DC, Holsen LM, Stern E, Rojas R, Ebbeling CB, et al. Effects of dietary glycemic index on brain regions related to reward and craving in men. Am J Clin Nutr. 2013;98:641-7.

Sun FH, Li C, Zhang YJ, Wong SH, Wang L. Effect of glycemic index of breakfast on energy intake at subsequent meal among healthy people: a meta-analysis. Nutrients. 2016;8:37-51.

Tremblay A, Doyon C, Sanchez M. Impact of yogurt on appetite control, energy balance, and body composition. Nutr Rev. 2015;73:23-7.

Gonzalez JT, Green BP, Brown MA, Rumbold PL, Turner LA, Stevenson EJ. Calcium ingestion suppresses appetite and produces acute overcompensation of energy intake independent of protein in healthy adults. J Nutr. 2015;145:476-82.

Gemechu T. Review on lactic acid bacteria function in milk fermentation and preservation. Afr J Food Sci.e. 2015;9:170-5.

Sanggaard KM, Holst JJ, Rehfeld JF, Sandstrom B, Raben A, Tholstrup T. Different effects of whole milk and a fermented milk with the same fat and lactose content on gastric emptying and postprandial lipaemia, but not on glycaemic response and appetite. Br J Nutr. 2004;92:447-59.

Reed DR, McDaniel AH. The human sweet tooth. Bmc Oral Health. 2006;6:S17-30. 31-6-S1-S17




Como Citar

Caferoglu, Z., & Aytekin Sahin, G. (2022). The effects of kefir in mixed meals on appetite and food intake: a randomized cross-over trial. Revista De Nutrição, 34, 1–11. Recuperado de