EVALUATION SPECIFIC HUMORAL IMMUNITY OF THE MEASLES VIRUS COMPARING FOUR DIAGNOSTIC TESTS
Keywords:
Measles, Plaque Reduction Neutralization Test, Enzyme-immune assay, Hemagglutination Inhibition TestAbstract
Sera samples from suspected measles cases received a/ter a mass vaccination campaign, showed 20.8%IgG negative for measles from two enzyme immune assays (EIA) (onecommercial/ Behring and another "home made" by CDC/Atlanta). These negative samples were tested by Hemagglutination Inhibition (HI) and Plaque Reduction Neutralization Test (PRNT} and the results were stratified according to age groups and vaccination status. The E/As (Behring and CDC) and HI presented 100% specificity and O. 90, O. 89, O. 93 sensibility, respectively, when compare d with PRNT. Children of 12 months or o ver had higher rates of false negative results in the EIAs. The true seronegative children were predominantly under 9 months of age. PRNT results were consistent with vaccination status in 81% of the children for whom data were available. PRNT should be used as a complementary test to EIA, in vaccination campaigns for the evaluation of seroconversion rates and for confirmation o/negative EIA results in clinicai samples.
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BELLINI, W.J., and Rota, P.A., 1995. Measles (rubeola) virus. ln: Lennette, E.H., Lennette, D.A. and Lennette, E.T. (Eds), Diagnostic procedures for virai, rickettsial, and chlamydial infections, American Public Health Association, 7'h edn, Washington, pp. 447-454.
BLACK, F.L. 1989a., Measles active and passive immunity in a worldwide perspective. Prog. Med. Virol. 36, 1-33.
BLACK, F.L., 1989b. Measles. ln: Evans, A.S. (Ed), Viral infections of humans. Epidemiology and contrai, 3ª ed, Plenum Press, New York, pp. 451-469.
CHEN, R.T., Markowitz, L.E., Albrecht, P., Stewart, J.A., Mofenson, L.M., Preblud, S.R., and Orenstein, W.A., 1990. Measles antibody: reevaluation of protective titers. J. lnfect. Ois. 162, 1036-1042.
CHUI, L.-W.L., Marusk, R.G., and Pabst, H.F., 1991. Measles vírus specific antibody in infants in a highly vaccinated society. J. Med. Viro!. 33, 199-204.
______, de Quadros, C.A., Olivé, J.M., Hersh, B.S., Strassburg, M.A., Henderson, D .. A., Brandling-Bennett, D., and Alleyne, G.A.O., 1996. Measles elimination in the Americas - Evolving strategies. JAMA 275, 224-229.
_____, de Sousa, V.A.U.F., Pannuti, C.S., Sumita, L.M., and Abrecht, P., 1991. Enzyme-linked immunosorbent assay (ELISA) for measles antibody. A comparison with hemagglutination inhibition, immunofluorescence and plaque neutralization tests. Rev. lnst. Med. Trop. São Paulo. 33 (1), 32-36.
DIAZ-ORTEGA, J.-L., Forsey, C.J., and Milstien, J., 1994. The relationship between dose and response of standard measles vaccines. Biologicals 22, 35-44.
ERDMAN, D.D., Anderson, L.J., Adams, D.R., Stewart, J.A., Markowitz, L.E., and Bellini, W.J., 1991. Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles vírus. J. Clin. Microbial. 29(7), 1466-1471.
FLEISS, J.L., 1981. Statistical Methods for Rates and Proportions. 2nd edn., John Wiley & Sons, New York, pp. 4-8.
FLETCHER, R.H., Fletcher, S.W., and Wagner, E.H., 1996. Clinicai Epidemiology- The Essentials. 3rd edn. Willians & Willians, Baltimore, pp. 43-75.
GERSHON, A.A. and Krugman,S., 1979. Measles Virus. ln: Lennette, E.H. and Schmidt, N.J. (Eds.), Diagnostic Procedures for Virai, Ricketsial and Chlamydial lnfections, 5th edn. American Public Health Association, Washington, DC. pp.665-693.
GRUPO Técnico do Sarampo, 1994. Sarampo: das Epidemias Rumo a Eliminação. Fundação Nacional de Saúde/ Ministério da Saúde. [Brasília].
HESKETH, L., Charlett, A., Farrington, P., Miller, E., Forsey, T., and Morgan-Capner, P., 1997. An evaluation of nine commercial-EIA kits for the detection ofmeasles specific lgG. J. Virai. Meth. 66, 51-59.
HUMMEL, K.B., Erdman, D.D., Heath, J., and Bellini, W.J., 1992. Baculovirus expression of the nucleoprotein gene of measles virus and utility of the recombinant protein in diagnostic enzyme immunoassays. J. Clin. Microbiol. 30, 2874-2880.
MODLIN, J.F., 1984. Measles Virus. ln: Belshe, R. (Ed.), Textbook ofHuman Virology. PSG Publishing Company, lnc., Littleton, Massachusetts, pp. 333-360.
NATES, SV., Rey, G.Y., Giordano, M.O., Zapata, M.T., Depetris, A., and Boshel, J., 1994. Modified serumneutralization assay for measles virus antibody detection. Res. Viro!. 145, 45-49.
NJA YOU, M., and Balia, A. 1990. Comparative study of peroxidase-labeled IgG and anti-IgG for detection of measles virus antigens. J. Viro!. Meth. 29, 91-96.
OLIVEIRA, S.A., Siqueira, M .. M., Mann, G.F., Costa, A.J., Almeida, M.T.C.N., Stavola, M.S., Tomasini, H., and Nascimento, J.P. 1996. Measles antibody prevalence after mass immunization campaign in Niterói, State of Rio de Janeiro, Brasil. Rev. Inst. Med. Trop. 38 (5), 355- 358.
RATNAM, S., Gadag, V., West, R., Burris, J., Oates, E., Stead, F, and Bouilianne, N. 1995. Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody. J. Clin. Microbiol. 33, 811-815.
ROSSIER, E., Miller, H., McCulloch, B., Sullivan, L., and Ward, K. 1991. Comparison of immunofluorescence and enzyme immunoassay for detection of measles specific immunoglobulins M antibody. J. Clin. Microbiol. 29, 1069-1071.
WALDMAN, E.A., and Camargo, M.C.C. 1996. Current status of measles in Brasil. 1980-1995. Virus Rev & Res. 1 (1/2), 67-74.
WEIGLE, K.A., Murph, MD., and Brunell, P.A. (1984). Enzyme-linked immunosorbent as say for evaluation of immunity to measles virus. J. Clin. Microbial. 19, 376-379.
WHITTLE, H.C., Rowland, M.G.M., Mann, G .. F., Lamb, W.H., and Lewis, R.A. 1984. Immunization of 4-6 month old Gambian infants with Edmonston-Zagreb measles vaccine. Lancet ii, 834-7.
WORLD Health Organization (1994). Laboratory diagnosis of measles infection and monitoring of measles immunization: Memorandum from a WHO meeting. Buli. WHO 72, 207-211.
