CHROMOSOME NUMBER, MEIOTIC BEHAVIOR AND POLLEN VIABILITY IN THREE SPECIES OF STYLOSANTHES SW. (LEGUMINOSAE-PAPILIONOIDEAE) NATIVE TO SOUTHERN BRAZIL
Keywords:
chromosomes, meiotic pairing, pollen, legumesAbstract
In this paper ten accessions of three species of leguminous forage Stylosanthes Sw. were analyzed with regard to the recount of the number of chromosomes, meiotic behavior and the percentage pollen viability by comparing three different dyes. In order to count the chromosome number, the meristematic regions of the roots were treated with pectinase-celullase 2% and stained with propionic orcein 2%. In the meiosis study and the estimation of pollen viability the flower-buds were fixed in absolute ethanol: chloroform: acetic acid (6:3:1). The material for meiosis was stained with lactopropionic orcein 1% and the pollen was stained with acetic orcein 1%, Alexander reactive and propionic carmine 1%. All accessions were diploids with 2n=2x=20 chromosomes. The meiotic behavior concerning microsporogenesis was considered normal with the meiotic index (MI) higher than 90%. In the estimation of pollen viability of these species the most dependable pigment was the Alexander reactive because it could differentiate through colors the cellular wall and the protoplasm.
Downloads
References
ALEXANDER, M.P.A . 1980. Versatile stain for pollen fungi, yeast and bacteria. Stain Technology, 55 (1): 13-18.
BARTZ, R. H., BISSANI, C.A. & SCHERER, E.E. 1995.
Recomendação de adubação e calagem para os estados do RS e SC. 3.ed. Passo Fundo-RS: EMBRAPA-CNTP, 221p.
BATTISTIN, A. 1981. Estudo biossistemático de diferentes taxons do gênero Stylosanthes Sw. (Leguminosae-Papilionoideae), São Paulo. 106p.. Dissertação (Mestrado em Genética e Melhoramento de Plantas). Escola Superior de Agricultura Luiz de Queiróz, Universidade de São Paulo.
BRANDÃO, M. 1992. Plantas do cerrado. Inf. Agrop., 16 (173): 36-39.
BURT, R. 1984. Natural variation in Stylosanthes. In: STACE, H. & EDYE, L.A. The Biology and Agronomy Stylosanthes. Australy: Academic press. P. 104-120.
CAMERON, D.F. 1967. Chromosome number and morphology of some introduced Stylosanthes species. Aust. J. Agric. Res, 18 : 375-379.
COSTA, N.M.S.& SCHWLTZE, K.R. 1990. Biogeografia de Stylosanthes capitata Vog. e S. guianensis Sw. var. paciflora. Pesq. Agrop. Bras., 25 (11): 1547-1554.
FERREIRA, N.B. & COSTA, M. B. S. 1979. O gênero Stylosanthes Sw no Brasil. Lavras: EPAMIG. 108 p.
GARDENER, C. J. 1984. The dinamics of Stylosanthes pasture. In: STACE, H. & EDYE, L. A. The biology and agronomy of Stylosantes. Australy: Academic press. p. 333-347.
GOLDBLATT, P. 1981. Citology and phylogeny of leguminosae. In: POLHILL, R.M. & RAVEN, R.H. Advances in legume systematics. Kew: Royal Botanic Garden. p. 427- 463.
MATTOS, A.C.F., BATTISTIN, A. & BIONDO E. 1998. Comportamento meiótico e estimativa da viabilidade de grãos de pólen em 10 genótipos de 3 espécies do gênero Stylosanthes Sw. nativas do sul do Brasil. Genetics and Molecular Biology, 21 (3) (supplement):194.
POLHILL, R.M. & RAVEN, R.H. 1981. Advances in legume systematics. Kew: Royal Botanic Garden, 425 p.
SOUSA, F.B. 1986. Avaliação de plantas forrageiras tropicais. In: SIMPÓSIO SOBRE PRODUÇÃO ANIMAL (3º). Campo Grande. Anais... Campo Grande-MS: Fundação Cargil. p. 59-63.
SPERONI, G. & ARTUCIO, P.I. 1994. El genero Stylosanthes (Fabaceae, Aeschynomeneae, Stylosanthinae) en Uruguay. Bol. Soc. Arg. Bot., 30 (1-2): 103-109.
STACE, H.M. & CAMERON, D.F. 1984. Cytogenetics and evolution of Stylosanthes. In: STACE, H.M. & EDYE. L.A. The biology and agronomy of Stylosanthes. Australy: Academic Press. p. 148-157.
THOMAS, D. 1984 . Global venture in Stylosanthes I south America. In: STACE, H.M. & EDYE, L.A. The biology and agronomy of Stylosanthes. Australy: Academic Press. p. 451- 464.
VIEIRA, M.L.C. 1988. Estudo citotaxonômico de espécies brasileiras do gênero Stylosanthes Sw. Piracicaba. 135p. Tese (Doutorado em Genética e Melhoramento de Plantas). Escola Superior de Agricultura Luiz de Queiróz Universidade de São Paulo.
VIEIRA, M.L.C.; AGUIAR-PERECIN, M L.R. & MARTINS, P.S. 1993. Cytotaxonomic study in twelve Brazilian taxa Stylosanthes Sw., Leguminosae. Cytologia, 58: 305-311.
